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Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10
- Source :
- Oncogene
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group UK, 2018.
-
Abstract
- Benefiting from more precise imaging and radiotherapy, patients with locoregionally nasopharyngeal carcinoma (NPC) have a significantly higher survival rate. Nonetheless, distant metastasis is still the predominant mode of failure. Advances in cancer research have highlighted that pathological angiogenesis is necessary for tumor metastasis by offering oxygen, nutrients, or cell metastatic conduits. MicroRNAs (miRNAs), a class of small noncoding RNAs, are increasingly implicated in modulation of angiogenesis in physiological and pathological conditions. Currently, we detected that miR-23a was highly enriched in NPC tissues at the metastatic or premetastatic stage, and its levels in NPC were associated with microvessel density. Subsequently, we proved that alteration of miR-23a expression modulated the growth, migration, and tube formation of HUVECs in vitro and affected the blood vessel outgrowth in the zebrafish model. Considering the possibility that extracellular miR-23a was horizontally transferred from CNE2 cells to HUVECs, we analyzed miR-23a encapsulated in exosomes, showing that overexpression of exosomal miR-23a in NPC promoted angiogenesis both in vitro and in vivo. Moreover, we provided evidences that miR-23a regulated angiogenesis by directly targeting testis-specific gene antigen (TSGA10). Taken together, our findings revealed that metastasis-associated miR-23a from NPC-derived exosomes plays an important role in mediating angiogenesis by targeting TSGA10.
- Subjects :
- 0301 basic medicine
Cancer Research
Angiogenesis
Biology
Exosomes
Article
Metastasis
Neovascularization
03 medical and health sciences
Mice
Cell Line, Tumor
microRNA
Genetics
medicine
Human Umbilical Vein Endothelial Cells
Animals
Humans
Neoplasm Metastasis
Molecular Biology
Zebrafish
Cell Proliferation
Regulation of gene expression
Tube formation
Nasopharyngeal Carcinoma
Neovascularization, Pathologic
Proteins
Nasopharyngeal Neoplasms
medicine.disease
Microvesicles
Up-Regulation
Gene Expression Regulation, Neoplastic
Cytoskeletal Proteins
MicroRNAs
030104 developmental biology
Nasopharyngeal carcinoma
Cancer research
Disease Progression
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 14765594 and 09509232
- Volume :
- 37
- Issue :
- 21
- Database :
- OpenAIRE
- Journal :
- Oncogene
- Accession number :
- edsair.doi.dedup.....c3c239c3d7c7152db82233e177e87ab4