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SDR7‐6, a short‐chain alcohol dehydrogenase/reductase family protein, regulates light‐dependent cell death and defence responses in rice
- Source :
- Molecular Plant Pathology
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Lesion mimic mutants resembling the hypersensitive response without pathogen attack are an ideal material to understand programmed cell death, the defence response, and the cross‐talk between defence response and development in plants. In this study, mic, a lesion mimic mutant from cultivar Yunyin treated with ethyl methanesulphonate (EMS), was screened. By map‐based cloning, a short‐chain alcohol dehydrogenase/reductase with an atypical active site HxxxK was isolated and designated as SDR7‐6. It functions as a homomultimer in rice and is localized at the endoplasmic reticulum. The lesion mimic phenotype of the mutant is light‐dependent. The mutant displayed an increased resistance response to bacterial blight, but reduced resistance to rice blast disease. The mutant and knockout lines showed increased reactive oxygen species, jasmonic acid content, antioxidant enzyme activity, and expression of pathogenicity‐related genes, while chlorophyll content was significantly reduced. The knockout lines showed significant reduction in grain size, seed setting rate, 1000‐grain weight, grain weight per plant, panicle length, and plant height. SDR7‐6 is a new lesion mimic gene that encodes a short‐chain alcohol dehydrogenase with atypical catalytic site. Disruption of SDR7‐6 led to cell death and had adverse effects on multiple agricultural characters. SDR7‐6 may act at the interface of the two defence pathways of bacterial blight and rice blast disease in rice.<br />SDR7‐6, a short‐chain alcohol dehydrogenase/reductase family protein, negatively regulates cell death and the defence response to rice bacterial blight, but positively regulates the defence response to Magnaporthe oryzae.
- Subjects :
- Hypersensitive response
Programmed cell death
SDR7‐6
Mutant
Soil Science
Plant Science
Reductase
short‐chain alcohol dehydrogenase/reductase
Lesion
Short Chain Dehydrogenase-Reductases
chemistry.chemical_compound
Gene Expression Regulation, Plant
medicine
Molecular Biology
Disease Resistance
Plant Diseases
Plant Proteins
Alcohol dehydrogenase
Cell Death
biology
rice
Jasmonic acid
food and beverages
Oryza
Original Articles
Molecular biology
Enzyme assay
Plant Leaves
chemistry
lesion mimic mutant
Mutation
biology.protein
Original Article
medicine.symptom
Oxidoreductases
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 13643703 and 14646722
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Molecular Plant Pathology
- Accession number :
- edsair.doi.dedup.....c3cb2e9e763c0627095478d0c80b67b9