A horseradish peroxidase study of the central projections of primary trigeminal neurons innervating the hard palate in the cat

The central projections of primary sensory neurons innervating the hard palate in the cat were studied by transganglionic transport of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP). Following injection of WGA-HRP in the incisive papilla terminal labeling was observed in all subdivisions of the sensory trigeminal nuclear complex. In the main sensory nucleus labeling was located in the dorsal part, especially in its most rostral portion. At the levels of nuclei oralis and interpolaris labeling was observed along the medial borders of the nuclei. In addition, at these levels distinct terminal labeling was located in patches within the trigeminal tract. In nucleus caudalis terminal labeling was confined to laminae I, II and V of the most rostral part of the nucleus. Some terminal labeling was observed also in the mid part of the solitary tract nucleus. After WGA-HRP injection in the posterior part of the hard palate a similar labeling pattern was found, but no labeling was observed in the solitary tract nucleus. The results in general indicate a relatively diffuse somatotopic organization of primary afferents innervating the palate. However, the somatotopic organization of palatine afferents within nucleus caudalis is at least partly consistent with the view that the central representation of the oral cavity is rotated 90 degrees to that of extraoral areas.