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The structural damages of lens crystallins induced by peroxynitrite and methylglyoxal, two causative players in diabetic complications and preventive role of lens antioxidant components
- Source :
- International Journal of Biological Macromolecules. 103:74-88
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Peroxynitrite (PON) and methylglyoxal (MGO), two diabetes-associated compounds, are believed to be important causative players in development of diabetic cataracts. In the current study, different spectroscopic methods, gel electrophoresis, lens culture and microscopic assessments were applied to examine the impact of individual, subsequent or simultaneous modification of lens crystallins with MGO and PON on their structure, oligomerization and aggregation. The protein modifications were confirmed with detection of the significantly increased quantity of carbonyl groups and decreased levels of sulfhydryl, tyrosine and tryptophan. Also, lens proteins modification with these chemical agents was accompanied with important structural alteration, oligomerization, disulfide/chromophore mediated protein crosslinking and important proteolytic instability. All these structural damages were more pronounced when the lens proteins were modified in the presence of both mentioned chemical agents, either in sequential or simultaneous manner. Ascorbic acid and glutathione, as the main components of lens antioxidant defense mechanism, were also capable to markedly prevent the damaging effects of PON and MGO on lens crystallins, as indicated by gel electrophoresis. The results of this study may highlight the importance of lens antioxidant defense system in protection of crystallins against the structural insults induced by PON and MGO during chronic hyperglycemia in the diabetic patients.
- Subjects :
- 0301 basic medicine
Antioxidant
medicine.medical_treatment
Ascorbic Acid
Biochemistry
Antioxidants
Diabetes Complications
Lens protein
Protein Aggregates
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Structural Biology
Crystallin
Peroxynitrous Acid
Lens, Crystalline
medicine
Animals
Protein Structure, Quaternary
Molecular Biology
Chemistry
Methylglyoxal
General Medicine
Glutathione
Pyruvaldehyde
Ascorbic acid
Crystallins
030104 developmental biology
medicine.anatomical_structure
Cytoprotection
Lens (anatomy)
030221 ophthalmology & optometry
Cattle
Protein Multimerization
Peroxynitrite
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 103
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....c40136620a03db6cd934557b1bf60e02