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IL-17 signalling restructures the nasal microbiome and drives dynamic changes following Streptococcus pneumoniae colonization
IL-17 signalling restructures the nasal microbiome and drives dynamic changes following Streptococcus pneumoniae colonization
- Source :
- BMC Genomics, BMC Genomics, Vol 18, Iss 1, Pp 1-20 (2017)
- Publication Year :
- 2017
- Publisher :
- BioMed Central, 2017.
-
Abstract
- Background The bacterial pathogen Streptococcus pneumoniae colonizes the nasopharynx prior to causing disease, necessitating successful competition with the resident microflora. Cytokines of the IL-17 family are important in host defence against this pathogen but their effect on the nasopharyngeal microbiome is unknown. Here we analyse the influence of IL-17 on the composition and interactions of the nasopharyngeal microbiome before and after pneumococcal colonization. Results Using a murine model and 16S rRNA profiling, we found that a lack of IL-17 signalling led to profound alterations in the nasal but not lung microbiome characterized by decreased diversity and richness, increases in Proteobacteria and reduction in Bacteroidetes, Actinobacteria and Acidobacteria. Following experimental pneumococcal nasal inoculation, animals lacking IL-17 family signalling showed increased pneumococcal colonization, though both wild type and knockout animals showed as significant disruption of nasal microbiome composition, with increases in the proportion of Proteobacteria, even in animals that did not have persistent colonization. Sparse correlation analysis of the composition of the microbiome at various time points after infection showed strong positive interactions within the Firmicutes and Proteobacteria, but strong antagonism between members of these two phyla. Conclusions These results show the powerful influence of IL-17 signalling on the composition of the nasal microbiome before and after pneumococcal colonization, and apparent lack of interspecific competition between pneumococci and other Firmicutes. IL-17 driven changes in nasal microbiome composition may thus be an important factor in successful resistance to pneumococcal colonization and potentially could be manipulated to augment host defence against this pathogen. Electronic supplementary material The online version of this article (10.1186/s12864-017-4215-3) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Lung microbiome
lcsh:QH426-470
Firmicutes
lcsh:Biotechnology
030106 microbiology
microbiome
Mucous membrane of nose
medicine.disease_cause
Ribotyping
Pneumococcal Infections
nasopharynx
Microbiology
Mice
03 medical and health sciences
lcsh:TP248.13-248.65
Streptococcus pneumoniae
Genetics
medicine
Animals
Colonization
Microbiome
Lung
Mice, Knockout
Innate immunity
Receptors, Interleukin-17
biology
Microbiota
Interleukin-17
Human microbiome
Genetic Variation
biology.organism_classification
medicine.disease
Mice, Inbred C57BL
lcsh:Genetics
Nasal Mucosa
Pneumococcal infections
030104 developmental biology
inflammation
Research Article
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Database :
- OpenAIRE
- Journal :
- BMC Genomics, BMC Genomics, Vol 18, Iss 1, Pp 1-20 (2017)
- Accession number :
- edsair.doi.dedup.....c42a60d97b06259e54586739cab0e91e