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Metabolic determinants in Listeria monocytogenes anaerobic listeriolysin O production
- Source :
- Archives of Microbiology
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Listeria monocytogenes is a human pathogen and a facultative anaerobe. To better understand how anaerobic growth affects L. monocytogenes pathogenesis, we first showed that anaerobic growth led to decreased growth and changes in surface morphology. Moreover, compared to aerobically grown bacteria, anaerobically grown L. monocytogenes established higher level of invasion but decreased intracellular growth and actin polymerization in cultured cells. The production of listeriolysin O (LLO) was significantly lower in anaerobic cultures-a phenotype observed in wild type and isogenic mutants lacking transcriptional regulators SigB or CodY or harboring a constitutively active PrfA. To explore potential regulatory mechanisms, we established that the addition of central carbon metabolism intermediates, such as acetate, citrate, fumarate, pyruvate, lactate, and succinate, led to an increase in LLO activity in the anaerobic culture supernatant. These results highlight the regulatory role of central carbon metabolism in L. monocytogenes pathogenesis under anaerobic conditions.
- Subjects :
- 0301 basic medicine
Virulence regulation
Tricarboxylic acid cycle
Bacterial Toxins
030106 microbiology
Mutant
Anaerobic metabolism
medicine.disease_cause
Biochemistry
Microbiology
Hemolysin Proteins
03 medical and health sciences
Bacterial Proteins
Listeria monocytogenes
Genetics
medicine
Humans
Listeriosis
Anaerobiosis
Molecular Biology
Heat-Shock Proteins
Original Paper
biology
Wild type
Listeriolysin O
General Medicine
biology.organism_classification
Citric acid cycle
Anaerobic exercise
Bacteria
Intracellular
Subjects
Details
- ISSN :
- 1432072X and 03028933
- Volume :
- 199
- Database :
- OpenAIRE
- Journal :
- Archives of Microbiology
- Accession number :
- edsair.doi.dedup.....c4425c949e9c76f98769523f1c2e8934