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Effects of MCLR exposure on sex hormone synthesis and reproduction-related genes expression of testis in male Rana nigromaculata
- Source :
- Environmental pollution (Barking, Essex : 1987). 236
- Publication Year :
- 2017
-
Abstract
- Microcystin-leucine-arginine (MCLR) is the most popular and toxic variant among microcystins, which can cause severe reproductive toxicity to animals. However, the mechanisms of reproductive toxicity induced by MCLR in amphibians are still not entirely clear. In the current study, toxicity mechanisms of MCLR on the reproductive system of male Rana nigromaculata followed by low concentration (0, 0.1, 1, and 10 μg/L) and short-term (0, 7, and 14 days) MCLR exposure were shown. It was observed that MCLR could be bioaccumulated in the testes of male frogs, and the theoretical bioaccumulation factor values were 0.24 and 0.19 exposed to 1 μg/L and 10 μg/L MCLR for 14 days, respectively. MCLR exposure significantly decreased testosterone (T) concentrations and increased estradiol (E2) concentrations exposed to 1 and 10 μg/L MCLR for 14 days. The mRNA levels of HSD17B3 were downregulated, and HSD17B1 and CYP19A1 mRNA expression levels were upregulated, respectively. Only 10 μg/L MCLR group showed significant induction of follicle-stimulating hormone (FSH) levels and cyclic adenosine monophosphate (cAMP) content. Moreover, AR and ESR1 mRNA expression levels were significantly upregulated exposed to 1 and 10 μg/L MCLR for 14 days, respectively. Our results suggested that low-concentration MCLR induced transcription changes of CYP19A1, HSD17B3, and HSD17B1 led to endocrine disorders, and caused interference of spermatogenesis by the decrease of T and abnormal gene expressions of AR and ESR1 in the testes of R. nigromaculata.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Microcystins
Ranidae
Health, Toxicology and Mutagenesis
Down-Regulation
Gene Expression
Microcystin-LR
010501 environmental sciences
Biology
Toxicology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Internal medicine
Testis
medicine
Animals
Cyclic adenosine monophosphate
Reproductive system
Gonadal Steroid Hormones
0105 earth and related environmental sciences
General Medicine
Sex hormone receptor
Pollution
Up-Regulation
030104 developmental biology
Endocrinology
chemistry
Toxicity
Reproductive toxicity
Spermatogenesis
Water Pollutants, Chemical
Hormone
Subjects
Details
- ISSN :
- 18736424
- Volume :
- 236
- Database :
- OpenAIRE
- Journal :
- Environmental pollution (Barking, Essex : 1987)
- Accession number :
- edsair.doi.dedup.....c44e7e68f23a83f07e5e8d4238d6158f