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Carbon monoxide-releasing molecule suppresses inflammatory and osteoclastogenic cytokines in nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via the heme oxygenase-1 pathway
- Source :
- International Journal of Molecular Medicine. 40:1591-1601
- Publication Year :
- 2017
- Publisher :
- Spandidos Publications, 2017.
-
Abstract
- Smoking is identified as a risk factor for periodontitis. Carbon monoxide (CO)-releasing molecule-3 (CORM-3) is a compound that has demonstrated anti-inflammatory effects in vitro and in vivo studies. The present study aimed to investigate the effects of CORM-3 on the expression of inflammatory and osteoclastogenic cytokines in human periodontal ligament cells (PDLCs) stimulated by nicotine and lipopolysaccharide (LPS). The cells were pretreated with CORM-3 and then cultured in medium in the presence of nicotine and LPS. The mRNA and protein expression levels of prostaglandin E2 (PGE2), cyclooxygenase-2 (COX‑2), osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL) and heme oxygenase-1 (HO-1) were evaluated using reverse transcription-quantitative polymerase chain reaction and western blot analysis. The mRNA and protein expression levels of these cytokines were also evaluated in PDLCs transiently transfected with HO-1 small interfering RNA (siRNA) in response to nicotine and LPS stimulation. CORM-3 attenuated the LPS- and nicotine-induced production of PGE2, COX-2 and RANKL in human PDLCs by releasing CO, and upregulated the expression of OPG. However, these effects of CORM-3 were abrogated when HO-1 siRNA was transiently transfected into the cells. These results demonstrate that CORM-3 exerts anti-inflammatory and anti-osteoclastogenic effects on nicotine- and LPS-stimulated human PDLCs via the HO-1 pathway, which suggests its promising potential for use in the treatment of inflammatory periodontal disease.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Nicotine
Small interfering RNA
Lipopolysaccharide
Cell Survival
Periodontal Ligament
Osteoclasts
Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Osteoprotegerin
Organometallic Compounds
Genetics
medicine
Humans
Bone Resorption
Periodontitis
Cells, Cultured
Carbon Monoxide
030206 dentistry
General Medicine
Transfection
Molecular biology
Cell biology
Heme oxygenase
030104 developmental biology
chemistry
Apoptosis
RANKL
biology.protein
Cytokines
Heme Oxygenase-1
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 1791244X and 11073756
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Medicine
- Accession number :
- edsair.doi.dedup.....c46b7a6a822e50b3f044d506135ffba2