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Intratracheally administered titanium dioxide or carbon black nanoparticles do not aggravate elastase-induced pulmonary emphysema in rats
- Source :
- BMC Pulmonary Medicine, Vol 12, Iss 1, p 38 (2012), BMC Pulmonary Medicine, BMC Pulmonary Medicine, BioMed Central, 2012, 12 (1), pp.38. ⟨10.1186/1471-2466-12-38⟩
- Publication Year :
- 2012
- Publisher :
- BMC, 2012.
-
Abstract
- Background Titanium dioxide (TiO2) and carbon black (CB) nanoparticles (NPs) have biological effects that could aggravate pulmonary emphysema. The aim of this study was to evaluate whether pulmonary administration of TiO2 or CB NPs in rats could induce and/or aggravate elastase-induced emphysema, and to investigate the underlying molecular mechanisms. Methods On day 1, Sprague-Dawley rats were intratracheally instilled with 25 U kg−1 pancreatic porcine elastase or saline. On day 7, they received an intratracheal instillation of TiO2 or CB (at 100 and 500 μg) dispersed in bovine serum albumin or bovine serum albumin alone. Animals were sacrificed at days 8 or 21, and bronchoalveolar lavage (BAL) cellularity, histological analysis of inflammation and emphysema, and lung mRNA expression of heme oxygenase-1 (HO-1), interleukin-1β (IL-1β), macrophage inflammatory protein-2, monocyte chemotactic protein-1, and matrix metalloprotease (MMP)-1, and -12 were measured. In addition, pulmonary MMP-12 expression was also analyzed at the protein level by immunohistochemistry. Results TiO2 NPs per se did not modify the parameters investigated, but CB NPs increased perivascular/peribronchial infiltration, and macrophage MMP-12 expression, without inducing emphysema. Elastase administration increased BAL cellularity, histological inflammation, HO-1, IL-1β and macrophage MMP-12 expression and induced emphysema. Exposure to TiO2 NPs did not modify pulmonary responses to elastase, but exposure to CB NPs aggravated elastase-induced histological inflammation without aggravating emphysema. Conclusions TiO2 and CB NPs did not aggravate elastase-induced emphysema. However, CB NPs induced histological inflammation and MMP-12 mRNA and protein expression in macrophages.
- Subjects :
- Male
Pathology
Interleukin-1beta
010501 environmental sciences
01 natural sciences
Bronchoalveolar Lavage
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Rats, Sprague-Dawley
Bovine serum albumin
Pancreatic elastase
Titanium
0303 health sciences
medicine.diagnostic_test
biology
Pancreatic Elastase
Elastase
respiratory system
medicine.anatomical_structure
Particles
Pulmonary Emphysema
Toxicity
Models, Animal
medicine.symptom
Research Article
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Inflammation
03 medical and health sciences
Soot
Matrix Metalloproteinase 12
medicine
Intubation, Intratracheal
Animals
COPD
030304 developmental biology
0105 earth and related environmental sciences
lcsh:RC705-779
Lung
business.industry
Monocyte
technology, industry, and agriculture
Pneumonia
lcsh:Diseases of the respiratory system
Rats
Bronchoalveolar lavage
biology.protein
Nanoparticles
[SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Occupational medicine
business
Heme Oxygenase-1
Subjects
Details
- Language :
- English
- ISSN :
- 14712466
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Pulmonary Medicine
- Accession number :
- edsair.doi.dedup.....c46e2f98e90e75ea4b432265f4978e4b