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Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis
- Source :
- Academica-e. Repositorio Institucional de la Universidad Pública de Navarra, instname, Fernandez-Fueyo, Elena; Ruiz-Duenas, Francisco J.; Ferreira, Patrica; Floudas, Dimitrios; HIbbett, David S.; Canessa, Paulo; et al.(2012). Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis. PNAS, 109(14). Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/5dm0n5pg, Zaguán. Repositorio Digital de la Universidad de Zaragoza, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Artículos CONICYT, CONICYT Chile, instacron:CONICYT, Academica-e: Repositorio Institucional de la Universidad Pública de Navarra, Universidad Pública de Navarra, Proceedings of the National Academy of Sciences, Digital.CSIC: Repositorio Institucional del CSIC, Consejo Superior de Investigaciones Científicas (CSIC), Proceedings of the National Academy of Sciences of the United States of America, 109(14), 5458-5463. National Academy of Sciences
- Publication Year :
- 2017
-
Abstract
- Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred to as white rot fungi. Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, Ceriporiopsis subvermispora, also depolymerizes lignin but may do so with relatively little cellulose degradation. To investigate the basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora and P. chrysosporium . Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium , respectively. In addition, the C. subvermispora genome contains at least seven genes predicted to encode laccases, whereas the P. chrysosporium genome contains none. We also observed expansion of the number of C. subvermispora desaturase-encoding genes putatively involved in lipid metabolism. Microarray-based transcriptome analysis showed substantial up-regulation of several desaturase and MnP genes in wood-containing medium. MS identified MnP proteins in C. subvermispora culture filtrates, but none in P. chrysosporium cultures. These results support the importance of MnP and a lignin degradation mechanism whereby cleavage of the dominant nonphenolic structures is mediated by lipid peroxidation products. Two C. subvermispora genes were predicted to encode peroxidases structurally similar to P. chrysosporium lignin peroxidase and, following heterologous expression in Escherichia coli , the enzymes were shown to oxidize high redox potential substrates, but not Mn 2+ . Apart from oxidative lignin degradation, we also examined cellulolytic and hemicellulolytic systems in both fungi. In summary, the C. subvermispora genetic inventory and expression patterns exhibit increased oxidoreductase potential and diminished cellulolytic capability relative to P. chrysosporium .
- Subjects :
- Selective ligninolysis
Molecular Sequence Data
Lignin
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Species Specificity
Manganese peroxidase
Phylogeny
030304 developmental biology
Chrysosporium
Laccase
0303 health sciences
Phanerochaete chrysosporium
Multidisciplinary
biology
030306 microbiology
Basidiomycota
Hydrolysis
Fungal genetics
Lignin peroxidase
Genomics
Biological Sciences
biology.organism_classification
3. Good health
chemistry
biology.protein
Phanerochaete
Ceriporiopsis subvermispora
Oxidation-Reduction
Peroxidase
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Database :
- OpenAIRE
- Journal :
- Academica-e. Repositorio Institucional de la Universidad Pública de Navarra, instname, Fernandez-Fueyo, Elena; Ruiz-Duenas, Francisco J.; Ferreira, Patrica; Floudas, Dimitrios; HIbbett, David S.; Canessa, Paulo; et al.(2012). Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis. PNAS, 109(14). Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/5dm0n5pg, Zaguán. Repositorio Digital de la Universidad de Zaragoza, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Artículos CONICYT, CONICYT Chile, instacron:CONICYT, Academica-e: Repositorio Institucional de la Universidad Pública de Navarra, Universidad Pública de Navarra, Proceedings of the National Academy of Sciences, Digital.CSIC: Repositorio Institucional del CSIC, Consejo Superior de Investigaciones Científicas (CSIC), Proceedings of the National Academy of Sciences of the United States of America, 109(14), 5458-5463. National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....c46fa1941a8f4df3b9c57a49dc5bdce7