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2,4-Dinitrophenol induces apoptosis in As4.1 juxtaglomerular cells through rapid depletion of GSH
- Source :
- Cell Biology International. 32:1536-1545
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- 2,4-Dinitrophenol (DNP) is an uncoupler of oxidative phosphorylation in mitochondria. Here, we investigated the in vitro effect of DNP on apoptosis and the involvement of reactive oxygen species (ROS) in As4.1 juxtaglomerular cell death. Dose- and time-dependent induction of apoptosis was evidenced by flow cytometric detection of sub-G1 DNA content and annexin V binding assay. The intracellular H(2)O(2) and O(2)(-) levels were markedly increased in DNP-treated cells. However, the reduction of intracellular H(2)O(2) level by Tiron and catalase did not prevent apoptosis induced by DNP. Moreover, DNP rapidly reduced intracellular GSH content in As4.1 cells. Taken together, apoptosis in DNP-treated As4.1 cells is correlated with the rapid change of intracellular GSH levels rather than ROS levels.
- Subjects :
- Time Factors
Down-Regulation
Apoptosis
chemical and pharmacologic phenomena
Oxidative phosphorylation
Biology
Oxidative Phosphorylation
Mice
chemistry.chemical_compound
Annexin
Cell Line, Tumor
medicine
Animals
Annexin A5
chemistry.chemical_classification
Reactive oxygen species
Tiron
Dose-Response Relationship, Drug
Uncoupling Agents
organic chemicals
Epithelial Cells
hemic and immune systems
DNA
Cell Biology
General Medicine
Glutathione
Juxtaglomerular cell
Molecular biology
Juxtaglomerular Apparatus
Mitochondria
Up-Regulation
Cell biology
Oxidative Stress
medicine.anatomical_structure
chemistry
2,4-Dinitrophenol
Reactive Oxygen Species
Intracellular
Subjects
Details
- ISSN :
- 10656995
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Cell Biology International
- Accession number :
- edsair.doi.dedup.....c4804821dbb2015459cc0b03c6b7a6ed