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Expression of a xylose-specific transporter improves ethanol production by metabolically engineered Zymomonas mobilis
- Source :
- Applied Microbiology and Biotechnology. 98:6897-6905
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- Zymomonas mobilis is a promising organism for biofuel production as it can produce ethanol from glucose at high rates. However, Z. mobilis does not natively ferment C5 sugars such as xylose. While it has been engineered to do so, the engineered strains do not metabolize these sugars at high rates. Previous research has identified some of the bottlenecks associated with xylose metabolism in Z. mobilis. In this work, we investigated transport as a possible bottleneck. In particular, we hypothesized that the slow uptake of xylose through the promiscuous Glf transporter may limit the efficiency of xylose metabolism in Z. mobilis. To test this hypothesis, we expressed XylE, the low-affinity xylose transporter from Escherichia coli, in a xylose-utilizing strain of Z. mobilis. Our results show that the expression of this pentose-specific transporter improves the rate of xylose utilization in Z. mobilis; however, this enhancement is seen only at high xylose concentrations. In addition, we also found that overexpression of the promiscuous Z. mobilis transporter Glf yielded similar results, suggesting that the transport bottleneck is not due to the specificity, but rather the capacity for sugar uptake.
- Subjects :
- Gene Expression
Xylose
medicine.disease_cause
Applied Microbiology and Biotechnology
Zymomonas mobilis
Metabolic engineering
chemistry.chemical_compound
Species Specificity
Xylose metabolism
Escherichia coli
medicine
Ethanol fuel
Zymomonas
Ethanol
Symporters
biology
Chemistry
Escherichia coli Proteins
Biological Transport
Transporter
General Medicine
biology.organism_classification
Glucose
Metabolic Engineering
Biochemistry
Fermentation
Biotechnology
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....c4863281d1512fa2b6db6ba9e10653b9
- Full Text :
- https://doi.org/10.1007/s00253-014-5812-6