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Both Talin-1 and Talin-2 correlate with malignancy potential of the human hepatocellular carcinoma MHCC-97 L cell
- Source :
- BMC Cancer
- Publisher :
- Springer Nature
-
Abstract
- Background Talin-1 (TLN-1) and TLN-2 are implicated in many cellular processes, but their roles in hepatocellular carcinoma (HCC) remain unclear. This study aimed to assess cell cycle distribution, anoikis, invasion and migration in human HCC MHCC-97 L cells. Methods MHCC-97 L cells, which highly express TLN-1, were transduced with TLN-1 shRNA (experimental group) or scramble shRNA (negative control group); non-transduced MHCC-97 L cells were used as blank controls. TLN-1 and TLN-2 mRNA and protein levels were detected by real-time RT-PCR and western blot, respectively. Then, cell cycle distribution and anoikis were assessed by flow cytometry. In addition, migration and invasion abilities were assessed using Transwell and cell scratch assays. Finally, a xenograft nude mouse model was established to further assess cell tumorigenicity. Results Compared with the blank and negative control groups, TLN-1/2 mRNA and protein levels were significantly reduced in the experiment group. TLN-1/2 knockdown cells showed significantly more cells in the G0/G1 phase (79.24 %) in comparison with both blank (65.36 %) and negative (62.69 %) control groups; conversely, less cells were found in G2/M and S phases in the experimental group compared with controls. Moreover, anoikis was enhanced (P
- Subjects :
- 0301 basic medicine
Talin
Pathology
medicine.medical_specialty
Cancer Research
Carcinoma, Hepatocellular
Cell
Flow cytometry
Cell cycle arrest
03 medical and health sciences
Mice
Nude mouse
Invasion
Cell Movement
Cell Line, Tumor
medicine
Genetics
Animals
Humans
Anoikis
Talin-1
Neoplasm Invasiveness
Hepatocellular carcinoma (HCC)
Migration
Cell Proliferation
Gene knockdown
biology
medicine.diagnostic_test
Cell growth
business.industry
Talin-2
Liver Neoplasms
Cell migration
Cell cycle
biology.organism_classification
Xenograft Model Antitumor Assays
Gene Expression Regulation, Neoplastic
030104 developmental biology
medicine.anatomical_structure
Oncology
Cancer research
business
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712407
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....c488872f686d5c53fc080db4ca7d64cc
- Full Text :
- https://doi.org/10.1186/s12885-016-2076-9