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Matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 in the respiratory tracts of human infants following paramyxovirus infection
- Source :
- Journal of medical virology. 79(4)
- Publication Year :
- 2007
-
Abstract
- Respiratory syncytial (RSV) and parainfluenza (PIV) viruses are primary causes of acute bronchiolitis and wheezing illnesses in infants and young children. To further understand inflammation in the airways following infection, we tested for the presence of matrix metalloproteinases (MMP) and natural tissue inhibitors of MMP (TIMP) in primary and established human cell lines, and in the nasopharyngeal secretions (NPS) of human infants infected with RSV or PIV. Using ELISA and multiplex-based assays, MMP-9 and TIMP-1 proteins were, respectively, detected in 66/67 and 67/67 NPS. During PIV or RSV infection TIMP-1 concentrations were associated with hypoxic bronchiolitis. TIMP-1 amounts were also negatively correlated with O2 saturation, and positively correlated with IL-6, MIP-1α, and G-CSF amounts following RSV infection. IL-6, MIP-1α, and G-CSF were negatively correlated with O2 saturation during RSV infection. Acute respiratory tract disease was not associated with MMP-9 protein/protease activity. Additional studies using real-time quantitative PCR suggested that MMP-9 mRNA copy numbers were elevated in normal human bronchial epithelial (NHBE) cells infected with RSV, while TIMP-1 and TIMP-2 were not increased. However, ELISA did not reveal MMP-9 protein in the NHBE cell culture supernatants. Hence, the data implied that airway epithelial cells were not the primary source of MMP or TIMP following paramyxovirus infection. Taken together, the data suggested that paramyxovirus infection perturbs MMP-9/TIMP-1 homeostasis that in turn may contribute to the severity of respiratory tract disease. J. Med. Virol. 79:447–456, 2007. © 2007 Wiley-Liss, Inc.
- Subjects :
- Male
Paramyxoviridae
Respiratory Mucosa
Respiratory Syncytial Virus Infections
Biology
medicine.disease_cause
Respirovirus Infections
Respirovirus
Virology
Cell Line, Tumor
Nasopharynx
Granulocyte Colony-Stimulating Factor
medicine
Humans
Respiratory system
Mononegavirales
Chemokine CCL4
Respiratory Tract Infections
Chemokine CCL3
Interleukin-6
Respiratory disease
Infant
Tissue Inhibitor of Metalloproteinases
respiratory system
Macrophage Inflammatory Proteins
medicine.disease
biology.organism_classification
Respiratory Syncytial Viruses
Oxygen
Infectious Diseases
Respiratory syncytial virus (RSV)
medicine.anatomical_structure
Matrix Metalloproteinase 9
Cell culture
Bronchiolitis
Immunology
Female
Respiratory tract
Subjects
Details
- ISSN :
- 01466615
- Volume :
- 79
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of medical virology
- Accession number :
- edsair.doi.dedup.....c49f37d9f7809ed44a3e9108ba155c63