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Expression of Nogo-66 receptor in human astrocytoma is correlated with tumor malignancy
- Source :
- Molecular biology reports. 39(3)
- Publication Year :
- 2010
-
Abstract
- Nogo-A is a myelin-associated neuronal growth inhibitory molecule in central nervous system after trauma. However, the physiological functions of Nogo-A in neural development and in healthy oligodendrocytes are largely unknown. In this study, we investigated the expression of Nogo-66 receptor (NgR) protein in 60 cases of human astrocytoma by Western blot RT-PCR and immunohistochemistry. The correlation between the expression of NgR and pathologic grades of astrocyoma was further analyzed. The results showed that the expression of NgR protein and NgR mRNA immunoreactivity score, were decreased markedly with the increasing pathological grades. Double immunostaining results showed that Nogo-A and NgR were colocalized at the interface of astrocytoma cells and extracellular matrix. Our results indicated that NgR may have inhibitory effects on tumor activity and Nogo-A may restrict migration of tumor cells via NgR.
- Subjects :
- Male
China
Blotting, Western
Receptors, Cell Surface
Biology
Astrocytoma
GPI-Linked Proteins
Extracellular matrix
Myelin
Western blot
Cell Movement
Nogo Receptor 1
mental disorders
Genetics
medicine
Humans
Receptor
Molecular Biology
DNA Primers
Analysis of Variance
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
medicine.disease
Immunohistochemistry
Extracellular Matrix
Blot
medicine.anatomical_structure
Immunology
Cancer research
Female
psychological phenomena and processes
Myelin Proteins
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 39
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....c4b39c37b08beeee4d24855410201d3c