Experimental monoarthritis

Bovine serum albumin (BSA) in complete Freund's adjuvant (CFA) was injected into the knee joints of previously BSA-sensitized guinea pigs and rabbits. The primary immune reaction to BSA prevemed secondary immune response to Trichinet(a Iarvae infection. We were unable to produce either eos(nophilia in the peripheral blood or antibodies against Trichinetla antigen (shown by complement fixation test). The additional injection of mediators of the inflammatory reaction or their precursors, e.g., complement component C5 and arachidonic acid, caused different histological pictures. C5 prodtrced a prolonged attire inflammatory phase with abundant neutrophils, whereas arachidonic acid did not significantly change the inflammatory response as compared to controls. The additional application of eosinophil-enriched preparation (EEP) caused a conspicuously reduced influx of monocytes/macrophages, a reduction of lymphocyte numbers, a prolonged influx of neutrophils, increased arylsnlfatase activity, earlier reduction of the inflam- matory process, and earlier onset of synoviocyte regeneration as compared to controls.