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An artificial chromosome for data storage
- Source :
- National Science Review
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- DNA digital storage provides an alternative for information storage with high density and long-term stability. Here, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval. The entire 254 kb sequence was 95.27% occupied by encoded data. The Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences. The stability was demonstrated by transmitting the data-carrying chromosome to the 100th generation. This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals, similar to a compact disc, with potential in economically massive data distribution.<br />A 254 kb artificial chromosome, storing two pictures and one video clip encoded with low-density parity-check codes and pseudo-random sequences, was manufactured and transmitted from generation to generation stably. The digital information residing in the chromosome was fast retrieved, tolerating base insertions and deletions in nanopore readout.
- Subjects :
- AcademicSubjects/SCI00010
Autonomously replicating sequence
Computer science
Computational biology
Human artificial chromosome
encoded DNA
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Data retrieval
Chromosome (genetic algorithm)
Information Science
030304 developmental biology
0303 health sciences
Multidisciplinary
business.industry
DNA storage
artificial chromosome
Replication (computing)
indel correction
chemistry
Computer data storage
synthetic biology
Nanopore sequencing
AcademicSubjects/MED00010
business
030217 neurology & neurosurgery
DNA
Research Article
Subjects
Details
- ISSN :
- 2053714X and 20955138
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- National Science Review
- Accession number :
- edsair.doi.dedup.....c51e41e55a0607f881339eefbf5d71fe