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Comprehensive quantitative analysis of Chinese patent drug YinHuang drop pill by ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 125:415-426
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- YinHuang drop pill (YHDP) is a new preparation, derived from the traditional YinHuang (YH) decoction. Since drop pills are one of the newly developed forms of Chinese patent drugs, not much research has been done regarding the quality and efficacy. This study aims to establish a comprehensive quantitative analysis of the chemical profile of YHDP. ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to identify 34 non-sugar small molecules including 15 flavonoids, 9 phenolic acids, 5 saponins, 1 iridoid, and 4 iridoid glycosides in YHDP samples, and 26 of them were quantitatively determined. Sugar composition of YHDP in terms of fructose, glucose and sucrose was examined via a high performance liquid chromatography-evaporative light scattering detector on an amide column (HPLC-NH2P-ELSD). Macromolecules were examined by high performance gel permeation chromatography coupled with ELSD (HPGPC-ELSD). The content of the drop pill's skeleton component PEG-4000 was also quantified via ultra-high performance liquid chromatography coupled with charged aerosol detector (UHPLC-CAD). The results showed that up to 73% (w/w) of YHDP could be quantitatively determined. Small molecules accounted for approximately 5%, PEG-4000 represented 68%, while no sugars or macromolecules were found. Furthermore, YHDP showed no significant differences in terms of daily dosage, compared to YinHuang granules and YinHuang oral liquid; however, it has a higher small molecules content compared to YinHuang lozenge.
- Subjects :
- Iridoid Glycosides
Sucrose
Iridoid
medicine.drug_class
Clinical Biochemistry
Analytical chemistry
Pharmaceutical Science
Decoction
02 engineering and technology
Mass spectrometry
01 natural sciences
Mass Spectrometry
Analytical Chemistry
Gel permeation chromatography
chemistry.chemical_compound
Chromatography detector
Drug Discovery
medicine
Chromatography, High Pressure Liquid
Spectroscopy
Chromatography
Drop (liquid)
010401 analytical chemistry
021001 nanoscience & nanotechnology
0104 chemical sciences
chemistry
0210 nano-technology
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....c5285a17d847e44f540d970cb2db137f