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Endosialin is expressed in high grade and advanced sarcomas: Evidence from clinical specimens and preclinical modeling
- Source :
- International Journal of Oncology.
- Publication Year :
- 2011
- Publisher :
- Spandidos Publications, 2011.
-
Abstract
- We previously surveyed the expression of endosialin/ CD248/TEM-1 by immunohistochemistry in human clinical specimens of sarcomas and documented expression in tumor cells, stromal cells and vasculature. In the present study, we completed a retrospective analysis of the diagnostic reports available for these same samples in order to identify high-grade and metastatic disease. Our results show that endosialin can be detected in advanced disease. We screened human sarcoma cell lines in vitro for endosialin expression and developed preclinical human xenograft models of disseminated sarcoma. We found that 22 out of 42 human sarcoma cell lines were positive for endosialin with a positive correlation between mRNA and protein levels. When implanted in vivo, endosialin was expressed at all sites of dissemination. These data provide clinical and preclinical evidence that endosialin can be detected in advanced sarcoma. These results demonstrate for the first time that endosialin is a suitable therapeutic target for poor prognosis and advanced disease.
- Subjects :
- Adult
Male
Cancer Research
medicine.medical_specialty
Pathology
Stromal cell
Adolescent
Transplantation, Heterologous
Endosialin
Young Adult
Antigens, CD
Antigens, Neoplasm
Cell Line, Tumor
Humans
Medicine
Child
Aged
Neoplasm Staging
Retrospective Studies
Oncogene
business.industry
Infant
Cancer
Sarcoma
Anatomical pathology
Middle Aged
medicine.disease
Molecular medicine
Gene Expression Regulation, Neoplastic
Oncology
Child, Preschool
Immunohistochemistry
Female
business
Subjects
Details
- ISSN :
- 17912423 and 10196439
- Database :
- OpenAIRE
- Journal :
- International Journal of Oncology
- Accession number :
- edsair.doi.dedup.....c53132c068c5eed74fc949d18068bc06