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Muscle Conditional Medium Reduces Intramuscular Adipocyte Differentiation and Lipid Accumulation through Regulating Insulin Signaling
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 18, Iss 8, p 1799 (2017), International Journal of Molecular Sciences; Volume 18; Issue 8; Pages: 1799
- Publication Year :
- 2017
- Publisher :
- MDPI, 2017.
-
Abstract
- Due to the paracrine effects of skeletal muscle, the lipid metabolism of porcine intramuscular (i.m.) preadipocytes was different from that of subcutaneous (s.c.) preadipocytes. To investigate the development of i.m. preadipocytes in vivo, the s.c. preadipocytes were cultured with muscle conditional cultured medium (MCM) for approximating extracellular micro-environment of the i.m. preadipocytes. Insulin signaling plays a fundamental role in porcine adipocyte differentiation. The expression levels of insulin receptor (INSR) and insulin-like growth factor 1 receptor (IGF-1R) in i.m. Preadipocytes were higher than that in s.c. preadipocytes. The effects of MCM on adipocyte differentiation, lipid metabolism and insulin signaling transdution were verified. MCM induced the apoptosis of s.c. preadipocytes but not of s.c. adipocytes. Moreover, MCM inhibited adipocyte differentiation at pre-differentiation and early stages of differentiation, while the expression levels of INSR and IGF-1R were increased. Furthermore, MCM treatment increased adipocyte lipolysis and fatty acid oxidation through induction of genes involved in lipolysis, thermogenesis, and fatty acid oxidation in mitochondria. Consistent with the above, treatment of s.c. adipocytes with MCM upregulated mitochondrial biogenesis. Taken together, MCM can approximate the muscle micro-environment and reduce intramuscular adipocyte differentiation and lipid accumulation via regulating insulin signaling.
- Subjects :
- 0301 basic medicine
Swine
medicine.medical_treatment
Apoptosis
Receptor, IGF Type 1
lcsh:Chemistry
extracellular micro-environment
chemistry.chemical_compound
Mice
Adipocyte
Adipocytes
Insulin
insulin receptor
lcsh:QH301-705.5
Spectroscopy
intramuscular preadipocytes
Organelle Biogenesis
Cell Differentiation
General Medicine
Computer Science Applications
medicine.anatomical_structure
Cellular Microenvironment
Adipogenesis
insulin-like growth factor 1 receptor
Signal Transduction
medicine.medical_specialty
Biology
Catalysis
Article
adipogenesis
Inorganic Chemistry
03 medical and health sciences
Paracrine signalling
Downregulation and upregulation
Internal medicine
3T3-L1 Cells
Paracrine Communication
medicine
Animals
Physical and Theoretical Chemistry
Muscle, Skeletal
Molecular Biology
Cell Proliferation
Growth factor
Organic Chemistry
Skeletal muscle
Lipid metabolism
porcine
Lipid Metabolism
Receptor, Insulin
Insulin receptor
030104 developmental biology
Endocrinology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Culture Media, Conditioned
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....c53ed1a1c4873c6c9ff734301fced0c7