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The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development
- Source :
- Nucleic Acids Research
- Publication Year :
- 2020
-
Abstract
- Methylation of nucleotides in ribosomal RNAs (rRNAs) is a ubiquitous feature that occurs in all living organisms. The formation of methylated nucleotides is performed by a variety of RNA-methyltransferases. Chloroplasts of plant cells result from an endosymbiotic event and possess their own genome and ribosomes. However, enzymes responsible for rRNA methylation and the function of modified nucleotides in chloroplasts remain to be determined. Here, we identified an rRNA methyltransferase, CMAL (Chloroplast MraW-Like), in the Arabidopsis chloroplast and investigated its function. CMAL is the Arabidopsis ortholog of bacterial MraW/ RsmH proteins and accounts to the N4-methylation of C1352 in chloroplast 16S rRNA, indicating that CMAL orthologs and this methyl-modification nucleotide is conserved between bacteria and the endosymbiont-derived eukaryotic organelle. The knockout of CMAL in Arabidopsis impairs the chloroplast ribosome accumulation and accordingly reduced the efficiency of mRNA translation. Interestingly, the loss of CMAL leads not only to defects in chloroplast function, but also to abnormal leaf and root development and overall plant morphology. Further investigation showed that CMAL is involved in the plant development probably by modulating auxin derived signaling pathways. This study uncovered the important role of 16S rRNA methylation mediated by CMAL in chloroplast ribosome biogenesis and plant development.
- Subjects :
- 0106 biological sciences
Chloroplasts
AcademicSubjects/SCI00010
Arabidopsis
Ribosome biogenesis
Plant Development
RRNA methylation
01 natural sciences
Ribosome
Methylation
Chloroplast ribosome
03 medical and health sciences
Gene Expression Regulation, Plant
RNA, Ribosomal, 16S
Genetics
Plastids
RNA, Messenger
Plastid
030304 developmental biology
0303 health sciences
biology
Nucleic Acid Enzymes
food and beverages
Methyltransferases
Ribosomal RNA
biology.organism_classification
Cell biology
Chloroplast
Plant Leaves
RNA, Plant
Ribosomes
010606 plant biology & botany
Subjects
Details
- ISSN :
- 13624962
- Volume :
- 48
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Nucleic acids research
- Accession number :
- edsair.doi.dedup.....c54ecf710f7ce4107baf4f804ddc4406