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Crystallization and preliminary X-ray diffraction data of the Fab fragment of a monoclonal antibody against apamin, a bee venom neurotoxin

Authors :
C, Devaux
M L, Defendini
P M, Alzari
C, Abergel
C, Granier
J C, Fontecilla-Camps
P M, Alzar
Laboratoire de biochimie [Marseille]
Aix-Marseille Université - Faculté de médecine (AMU MED)
Aix Marseille Université (AMU)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Immunologie structurale
Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Laboratoire de Cristallographie et de Cristallisation des Macromolécules Biologiques [Marseille]
This work was supported by institutional grants from INSERM and CNRS.
Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Source :
FEBS Letters, FEBS Letters, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩, FEBS Letters, Wiley, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩
Publication Year :
1991
Publisher :
HAL CCSD, 1991.

Abstract

International audience; Fab fragments of anti-apamin monoclonal antibodies have been purified to homogeneity and crystallized. The crystals belong to the monoclinic space group P21 with cell dimensions a = 99.0 +/- 0.3 A, b = 137.1 +/- 0.4 A, c = 76.0 +/- 0.2 A and beta = 92.9 +/- 0.9 degrees. They most likely contain four molecules in the asymmetric unit (Vm = 2.39 A3/Da). The possibility of the existence of non-crystallographic symmetry is discussed.

Details

Language :
English
ISSN :
00145793 and 18733468
Database :
OpenAIRE
Journal :
FEBS Letters, FEBS Letters, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩, FEBS Letters, Wiley, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩
Accession number :
edsair.doi.dedup.....c5532cbbcd7c8a84f78d65bd2492110c