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Crystallization and preliminary X-ray diffraction data of the Fab fragment of a monoclonal antibody against apamin, a bee venom neurotoxin
- Source :
- FEBS Letters, FEBS Letters, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩, FEBS Letters, Wiley, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩
- Publication Year :
- 1991
- Publisher :
- HAL CCSD, 1991.
-
Abstract
- International audience; Fab fragments of anti-apamin monoclonal antibodies have been purified to homogeneity and crystallized. The crystals belong to the monoclinic space group P21 with cell dimensions a = 99.0 +/- 0.3 A, b = 137.1 +/- 0.4 A, c = 76.0 +/- 0.2 A and beta = 92.9 +/- 0.9 degrees. They most likely contain four molecules in the asymmetric unit (Vm = 2.39 A3/Da). The possibility of the existence of non-crystallographic symmetry is discussed.
- Subjects :
- Monoclonal antibody
Electrophoresis
medicine.drug_class
Stereochemistry
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Biophysics
Venom
MESH: Electrophoresis
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Apamin
Biochemistry
law.invention
MESH: Antibodies, Monoclonal
Immunoglobulin Fab Fragments
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
X-Ray Diffraction
Structural Biology
law
Genetics
medicine
[CHIM.CRIS]Chemical Sciences/Cristallography
Fab purification
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Crystallization
Bovine serum albumin
Molecular Biology
X-ray crystallography
030304 developmental biology
MESH: Crystallization
0303 health sciences
biology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
MESH: X-Ray Diffraction
Antibodies, Monoclonal
Fast protein liquid chromatography
Cell Biology
MESH: Immunoglobulin Fab Fragments
chemistry
biology.protein
2-Methyl-2,4-pentanediol
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
030217 neurology & neurosurgery
MESH: Apamin
Monoclinic crystal system
Subjects
Details
- Language :
- English
- ISSN :
- 00145793 and 18733468
- Database :
- OpenAIRE
- Journal :
- FEBS Letters, FEBS Letters, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩, FEBS Letters, Wiley, 1991, 286 (1-2), pp.64-66. ⟨10.1016/0014-5793(91)80941-u⟩
- Accession number :
- edsair.doi.dedup.....c5532cbbcd7c8a84f78d65bd2492110c