Back to Search
Start Over
A single-cell atlas of mouse olfactory bulb chromatin accessibility
- Source :
- Journal of Genetics and Genomics. 48:147-162
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Olfaction, the sense of smell, is a fundamental trait crucial to many species. The olfactory bulb (OB) plays pivotal roles in processing and transmitting odor information from the environment to the brain. The cellular heterogeneity of the mouse OB has been studied using single-cell RNA sequencing. However, the epigenetic landscape of the mOB remains mostly unexplored. Herein, we apply single-cell assay for transposase-accessible chromatin sequencing to profile the genome-wide chromatin accessibility of 9,549 single cells from the mOB. Based on single-cell epigenetic signatures, mOB cells are classified into 21 clusters corresponding to 11 cell types. We identify distinct sets of putative regulatory elements specific to each cell cluster from which putative target genes and enriched potential functions are inferred. In addition, the transcription factor motifs enriched in each cell cluster are determined to indicate the developmental fate of each cell lineage. Our study provides a valuable epigenetic data set for the mOB at single-cell resolution, and the results can enhance our understanding of regulatory circuits and the therapeutic capacity of the OB at the single-cell level.
- Subjects :
- Epigenomics
Cell
Olfaction
Computational biology
Biology
Epigenesis, Genetic
Mice
03 medical and health sciences
0302 clinical medicine
Genetics
medicine
Animals
Cluster Analysis
Cell Lineage
Gene Regulatory Networks
Regulatory Elements, Transcriptional
Epigenetics
Nucleotide Motifs
Molecular Biology
Transcription factor
Gene
030304 developmental biology
0303 health sciences
RNA
Olfactory Bulb
Chromatin
Olfactory bulb
medicine.anatomical_structure
Viruses
Single-Cell Analysis
030217 neurology & neurosurgery
Transcription Factors
Subjects
Details
- ISSN :
- 16738527
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Journal of Genetics and Genomics
- Accession number :
- edsair.doi.dedup.....c576ad339e3bd29469b4758db6b97e44