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Immunohistochemical investigation of hepatitis B virus associated antigens, HLA antigens and lymphocyte subsets in type B chronic hepatitis
Immunohistochemical investigation of hepatitis B virus associated antigens, HLA antigens and lymphocyte subsets in type B chronic hepatitis
- Source :
- Gastroenterologia Japonica. 25(1)
- Publication Year :
- 1990
-
Abstract
- HLA antigens, hepatitis B virus (HBV)-associated antigens and lymphocyte subsets in liver tissue from 35 patients with HBs antigenemia were studied using an immunoperoxidase double staining method and immunoelectron microscopy in order to clarify the immune mechanism of hepatocyte lysis in type B hepatitis. Immune light and electron microscopy using monoclonal antibodies to lymphocyte subsets revealed that infiltrating lymphocytes in the areas of piecemeal necrosis and focal necrosis were predominantly CD8-positive, showing direct contact with hepatocytes. In contrast, CD4(+) cells were infrequently observed in necrotizing inflammatory lesions. HLA-A,B,C antigens were mainly found on hepatocytes in areas of piecemeal necrosis and focal necrosis, in association with CD8(+) lymphocyte infiltration. HLA-DR antigens were demonstrated on a few hepatocytes in the same lesions. In cases of CAH with serum HBeAg positive, HLA-A,B,C, antigens and HBV antigens simultaneously demonstrated on the same hepatocytes. Especially, hepatocytes expressing both HLA-A,B,C antigen and HBsAg on the plasma membrane showed direct contact with CD8(+)lymphocytes. This finding fulfilled the morphological requirements for HBsAg as a target antigen. On the other hand, HBcAg was hardly demonstrated in the liver cell membrane but was demonstrated mainly in the cytoplasm. Compared with the nuclear localization of HBcAg in cases of NSR, cytoplasmic localization of this antigen may be associated with membranous expression of new antigens induced by HBV infection.
- Subjects :
- Piecemeal necrosis
HBsAg
Immunoelectron microscopy
Biology
medicine.disease_cause
Hepatitis B Antigens
Immunoenzyme Techniques
Leukocyte Count
Necrosis
Antigen
HLA Antigens
medicine
Humans
Lymphocytes
Pan-T antigens
Hepatitis, Chronic
Hepatitis B virus
Hepatitis
Gastroenterology
virus diseases
medicine.disease
Hepatitis B
digestive system diseases
HBcAg
Microscopy, Electron
Liver
Immunology
Chronic Disease
Subjects
Details
- ISSN :
- 04351339
- Volume :
- 25
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Gastroenterologia Japonica
- Accession number :
- edsair.doi.dedup.....c5851970372ced0dc1b4395e04193c26