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Overexpression of eIF-5A2 in mice causes accelerated organismal aging by increasing chromosome instability
- Source :
- BMC Cancer, Vol 11, Iss 1, p 199 (2011), BMC Cancer
- Publication Year :
- 2011
- Publisher :
- BMC, 2011.
-
Abstract
- Background: Amplification of 3q26 is one of the most frequent genetic alterations in many human malignancies. Recently, we isolated a novel oncogene eIF-5A2 within the 3q26 region. Functional study has demonstrated the oncogenic role of eIF-5A2 in the initiation and progression of human cancers. In the present study, we aim to investigate the physiological and pathological effect of eIF-5A2 in an eIF-5A2 transgenic mouse model.Methods: An eIF-5A2 transgenic mouse model was generated using human eIF-5A2 cDNA. The eIF-5A2 transgenic mice were characterized by histological and immunohistochemistry analyses. The aging phenotypes were further characterized by wound healing, bone X-ray imaging and calcification analysis. Mouse embryo fibroblasts (MEF) were isolated to further investigate molecular mechanism of eIF-5A2 in aging.Results: Instead of resulting in spontaneous tumor formation, overexpression of eIF-5A2 accelerated the aging process in adult transgenic mice. This included decreased growth rate and body weight, shortened life span, kyphosis, osteoporosis, delay of wound healing and ossification. Investigation of the correlation between cellular senescence and aging showed that cellular senescence is not required for the aging phenotypes in eIF-5A2 mice. Interestingly, we found that activation of eIF-5A2 repressed p19 level and therefore destabilized p53 in transgenic mouse embryo fibroblast (MEF) cells. This subsequently allowed for the accumulation of chromosomal instability, such as errors in cell dividing during metaphase and anaphase. Additionally, a significantly increase in number of aneuploidy cells (p < 0.05) resulted from an increase in the incidences of misaligned and lagging chromosomal materials, anaphase bridges, and micronuclei in the transgenic mice.Conclusion: These observations suggest that eIF-5A2 mouse models could accelerate organismal aging by increasing chromosome instability. © 2011 Chen et al; licensee BioMed Central Ltd.<br />published_or_final_version
- Subjects :
- Genetically modified mouse
Male
Pathology
medicine.medical_specialty
Cancer Research
Gene Expression
Mice, Transgenic
Biology
environment and public health
lcsh:RC254-282
Bone and Bones
Mice
EIF-5A2 aging
Peptide Initiation Factors
Pregnancy
oncogene
Chromosome instability
Chromosomal Instability
medicine
Genetics
Animals
Fibroblast
Metaphase
Cellular Senescence
Anaphase
Oncogene
aging
Gene Expression Regulation, Developmental
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Cell biology
Radiography
transgenic mouse
medicine.anatomical_structure
Cell Transformation, Neoplastic
Phenotype
Oncology
eIF-5A2
Female
Stem cell
chromosome instability
Cell aging
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712407
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....c589b9990a57d6a7430512242d7040bd