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Asparagus racemosus bZIP transcription factor-regulated squalene epoxidase (ArSQE) promotes germination and abiotic stress tolerance in transgenic tobacco
- Source :
- Plant Science. 290:110291
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- A. racemosus is a rich source of pharmacologically active steroidal saponins. Most of the studies are related to its chemistry and pharmacology, but the pathway involved in the biosynthesis of steroidal saponin is not much emphasized. Squalene epoxidase acts as a rate-limiting enzyme in this biosynthesis. In this study, we have selected root specific squalene epoxidase ArSQE from A. racemosus for its characterization. ArSQE was able to complement ergosterol auxotrophy in erg1 yeast mutants. Mutants were sensitive to the antifungal drug terbinafine, whereas ArSQE complementation made them tolerant to the same drug. ArSQE plays a significant role in early germination in transgenic tobacco. The transgenic tobacco seedlings overexpressing ArSQE were tolerant to terbinafine and abiotic stress. Expression analysis of transcripts in ArSQE transgenic lines suggests that it mostly affects ABA, GA, stress, and sterol related functions in transgenic tobacco. Further, root specific MeJA responsive A. racemosus bZIP transcription factors (TFs), ArTGA1 and ArTGA2, were identified that bind to MeJA responsive cis-element present in the promoter region of ArSQE. Characterization of ArSQE of A. racemosus provides new information about its regulation through MeJA responsive bZIP TF along with its role in the development and abiotic stress response in transgenic tobacco.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Squalene monooxygenase
Transgene
Auxotrophy
Antifungal drug
Germination
Plant Science
Biology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
Stress, Physiological
Tobacco
Genetics
Asparagus racemosus
Amino Acid Sequence
Transcription factor
Phylogeny
Plant Proteins
Ergosterol
Abiotic stress
General Medicine
Plants, Genetically Modified
biology.organism_classification
Basic-Leucine Zipper Transcription Factors
030104 developmental biology
Squalene Monooxygenase
Biochemistry
chemistry
Asparagus Plant
Sequence Alignment
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 01689452
- Volume :
- 290
- Database :
- OpenAIRE
- Journal :
- Plant Science
- Accession number :
- edsair.doi.dedup.....c5a13cb197ea41e1c6100d1178474e73