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Petroleum extract of Farfarae Flos alleviates nasal symptoms by regulating the Th1-Th2 cytokine balance in a mouse model of Allergic Rhinitis
- Source :
- International Journal of Medical Sciences
- Publication Year :
- 2021
- Publisher :
- Ivyspring International Publisher, 2021.
-
Abstract
- Farfarae Flos is a traditional Chinese medicine that has long been used to treat allergies. In this study, we aimed to investigate the effect of a petroleum extract of Farfarae Flos (PEFF) in a mouse model of allergic rhinitis (AR) and to explore the underlying molecular mechanisms of action. An animal model of AR was established by sensitization and challenge of BALB/c mice with ovalbumin (OVA). PEFF was administered intranasally and AR nasal symptoms were assessed on a semi-quantitative scale according to the frequencies of nose rubbing and sneezing and the degree of rhinorrhea. The mechanism of action of PEFF was evaluated by histological analysis of nasal mucosa architecture and inflammatory status; ELISA-based quantification of serum OVA-specific IgE, interferon-γ (IFN-γ), and interleukin-4 (IL-4) concentrations; and immunohistochemical and western blot analysis of T-bet and GATA3 protein expression in nasal mucosa and spleen tissues. The results showed intranasal administration of PEFF alleviated AR symptom scores and reduced both the infiltration of inflammatory cells and tissue damage in the nasal mucosa. PEFF significantly decreased serum concentrations of OVA-specific IgE (P
- Subjects :
- Allergic Rhinitis
Allergy
Ovalbumin
medicine.medical_treatment
Mucous membrane of nose
Tussilago
Flowers
Pharmacology
Immunoglobulin E
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Animals
Humans
Th1-Th2
Th1-Th2 Balance
Cytokine
Sensitization
Administration, Intranasal
Mice, Inbred BALB C
rhinorrhea
biology
business.industry
Plant Extracts
General Medicine
medicine.disease
Rhinitis, Allergic
Disease Models, Animal
Nasal Mucosa
medicine.anatomical_structure
Petroleum
Gene Expression Regulation
biology.protein
Farfarae Flos
030211 gastroenterology & hepatology
Nasal administration
Female
IgE
medicine.symptom
business
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 14491907
- Volume :
- 18
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- International Journal of Medical Sciences
- Accession number :
- edsair.doi.dedup.....c5b984e953126dc7539fbeeec2437198