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Single-cell analysis of diverse immune phenotypes in malignant pleural effusion
- Source :
- Nature Communications, Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- The complex interactions among different immune cells have important functions in the development of malignant pleural effusion (MPE). Here we perform single-cell RNA sequencing on 62,382 cells from MPE patients induced by non-small cell lung cancer to describe the composition, lineage, and functional states of infiltrating immune cells in MPE. Immune cells in MPE display a number of transcriptional signatures enriched for regulatory T cells, B cells, macrophages, and dendritic cells compared to corresponding counterparts in blood. Helper T, cytotoxic T, regulatory T, and T follicular helper cells express multiple immune checkpoints or costimulatory molecules. Cell-cell interaction analysis identifies regulatory B cells with more interactions with CD4+ T cells compared to CD8+ T cells. Macrophages are transcriptionally heterogeneous and conform to M2 polarization characteristics. In addition, immune cells in MPE show the general up-regulation of glycolytic pathways associated with the hypoxic microenvironment. These findings show a detailed atlas of immune cells in human MPE and enhance the understanding of potential diagnostic and therapeutic targets in advanced non-small cell lung cancer.<br />Detailed immune cell subset identification in human lung (malignant pleural effusion) MPE is important for diagnosis and prognosis. Here the authors use single cell RNA sequencing to identify immune cell types within MPE and show differential gene expression compared to circulating immune cells in blood.
- Subjects :
- Cancer microenvironment
Male
Lung Neoplasms
animal diseases
Science
T-Lymphocytes
Regulatory B cells
General Physics and Astronomy
chemical and pharmacologic phenomena
Kaplan-Meier Estimate
Biology
Article
General Biochemistry, Genetics and Molecular Biology
Immunophenotyping
Immune system
Single-cell analysis
Carcinoma, Non-Small-Cell Lung
Tumor Microenvironment
medicine
Humans
Cytotoxic T cell
Malignant pleural effusion
RNA-Seq
Aged
B-Lymphocytes
Multidisciplinary
Gene Expression Profiling
Macrophages
Immunosurveillance
RNA
General Chemistry
biochemical phenomena, metabolism, and nutrition
Middle Aged
medicine.disease
Phenotype
Pleural Effusion, Malignant
Gene Expression Regulation, Neoplastic
Lymphocyte differentiation
Cancer research
bacteria
Female
Single-Cell Analysis
Non-small-cell lung cancer
CD8
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....c5e3992bbe15fc8985a75eece72ac64f