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Rotating-disc micro-solid phase extraction of F2-isoprostanes from maternal and cord plasma by using oxidized buckypaper as sorbent membrane
- Source :
- Journal of chromatography. A. 1586
- Publication Year :
- 2018
-
Abstract
- This paper describes the development of an original micro-solid phase extraction device and its evaluation for the isolation of F2-isoprostanes (F2-IsoPs) from cord and maternal plasma samples. The unit is very simple and consists in a rotating disc (1.8 cm diameter) of oxidized buckypaper (BP), enwrapped in a polypropylene mesh pouch. Even if the selected F2-IsoPs have logP and pKa values that make them suitable candidates for their sorption on BP, several parameters were optimized to maximize recoveries: time of adsorption and desorption; stirring speed; volume, pH and ionic strength of the sample; type, volume, and fractions of the elution solvent; oxidation grade of BP. Among all, the last one was crucial in affecting extraction yields because of the analyte interactions with polar functionalities, introduced by a preliminary oxidative acid treatment. The investigation established the optimal oxidation time and highlighted the pros and cons of the acid activation step. All extracts were analyzed by means of liquid chromatography-tandem mass spectrometry (LC–MS/MS). Validation was performed according to the main FDA guidelines for bioanalytical methods. Depending on the spike level and analyte, recoveries ranged between 30 and 120% with precision and accuracy values lower than 20%. Quantitative analysis was accomplished by matrix-matched calibration curves whose determination coefficients were higher than 0.95. Lower limit of quantitation (LLOQ) spanned the range 2.45–6.77 μg L−1. The validated method was applied to the analysis of eight pairs of mother/child plasma samples, revealing the presence of 8-iso-15-keto-PGF2α and 8-iso-PGE2 at a concentration of about 10 μg L−1 in most cord plasma samples of preterm newborns.
- Subjects :
- Paper
Analyte
Buckypaper
Calibration curve
Biological samples
Carbon nanotubes
LC–MS
Sample preparation
Solid phase extraction
Adsorption
F2-Isoprostanes
Female
Fetal Blood
Humans
Infant, Newborn
Limit of Detection
Nanotubes, Carbon
Pregnancy
Solid Phase Extraction
Solvents
Tandem Mass Spectrometry
010402 general chemistry
01 natural sciences
Biochemistry
Carbon nanotube
Analytical Chemistry
Liquid chromatography–mass spectrometry
Biological sample
Nanotubes
Chromatography
Chemistry
Elution
010401 analytical chemistry
Organic Chemistry
Extraction (chemistry)
Infant
General Medicine
Newborn
biological samples
buckypaper
carbon nanotubes
sample preparation
solid phase extraction
analytical chemistry
biochemistry
organic chemistry
Carbon
0104 chemical sciences
Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA
Quantitative analysis (chemistry)
Subjects
Details
- ISSN :
- 18733778
- Volume :
- 1586
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. A
- Accession number :
- edsair.doi.dedup.....c5ed5a0021a839e66a63b61031e45cb5