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Detection of colorectal cancer in urine using DNA methylation analysis

Authors :: Geert Kazemier
Idris Bahce
I. Martin
Nina R. Sluiter
M. A. van de Wiel
Sander Bach
I. Paulis
Renske D.M. Steenbergen
M. Tibbesma
Jurriaan B. Tuynman
Mathematics
Surgery
Epidemiology and Data Science
APH - Methodology
CCA - Imaging and biomarkers
Amsterdam Gastroenterology Endocrinology Metabolism
Pulmonary medicine
Source :: Scientific Reports, 11:2363, 1-11. Nature Publishing Group, Bach, S, Paulis, I, Sluiter, N R, Tibbesma, M, Martin, I, van de Wiel, M A, Tuynman, J B, Bahce, I, Kazemier, G & Steenbergen, R D M 2021, ' Detection of colorectal cancer in urine using DNA methylation analysis ', Scientific Reports, vol. 11, no. 1, 2363, pp. 2363 . https://doi.org/10.1038/s41598-021-81900-6, https://doi.org/10.1038/s41598-021-81900-6, Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021), Bach, S, Paulis, I, Sluiter, N R, Tibbesma, M, Martin, I, van de Wiel, M A, Tuynman, J B, Bahce, I, Kazemier, G & Steenbergen, R D M 2021, ' Detection of colorectal cancer in urine using DNA methylation analysis ', Scientific Reports, vol. 11, 2363, pp. 1-11 . https://doi.org/10.1038/s41598-021-81900-6, Scientific Reports, 11(1):2363. Nature Publishing Group
Publication Year :: 2021
Abstract: Colorectal cancer (CRC) is the second leading cause for cancer-related death globally. Clinically, there is an urgent need for non-invasive CRC detection. This study assessed the feasibility of CRC detection by analysis of tumor-derived methylated DNA fragments in urine. Urine samples, including both unfractioned and supernatant urine fractions, of 92 CRC patients and 63 healthy volunteers were analyzed for DNA methylation levels of 6 CRC-associated markers (SEPT9, TMEFF2, SDC2, NDRG4, VIM and ALX4). Optimal marker panels were determined by two statistical approaches. Methylation levels of SEPT9 were significantly increased in urine supernatant of CRC patients compared to controls (p SEPT9 and SDC2 was able to detect up to 70% of CRC cases in urine supernatant at 86% specificity. First evidence is provided for CRC detection in urine by SEPT9 methylation analysis, which combined with SDC2 allows for an optimal differentiation between CRC patients and controls. Urine therefore provides a promising liquid biopsy for non-invasive CRC detection.