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Mesenchymal proteases and tissue fluidity remodel the extracellular matrix during airway epithelial branching in the embryonic avian lung
- Source :
- Development
- Publication Year :
- 2019
- Publisher :
- The Company of Biologists Ltd, 2019.
-
Abstract
- Reciprocal epithelial-mesenchymal signaling is essential for morphogenesis, including branching of the lung. In the mouse, mesenchymal cells differentiate into airway smooth muscle that wraps around epithelial branches, but this contractile tissue is absent from the early avian lung. Here, we found that branching morphogenesis in the embryonic chicken lung requires extracellular matrix (ECM) remodeling driven by reciprocal interactions between the epithelium and mesenchyme. Before branching, the basement membrane wraps the airway epithelium as a spatially uniform sheath. After branch initiation, however, the basement membrane thins at branch tips; this remodeling requires mesenchymal expression of matrix metalloproteinase-2, which is necessary for branch extension but not branch initiation. As branches extend, tenascin-C (TNC) accumulates in the mesenchyme several cell diameters away from the epithelium. Despite its pattern of accumulation, TNC is expressed exclusively by epithelial cells. Branch extension coincides with deformation of adjacent mesenchymal cells, which correlates with an increase in mesenchymal fluidity at branch tips that may transport TNC away from the epithelium. These data reveal novel epithelial-mesenchymal interactions that direct ECM remodeling during airway branching morphogenesis.
- Subjects :
- Mesenchyme
Morphogenesis
Chick Embryo
Respiratory Mucosa
Biology
Basement Membrane
Extracellular matrix
Mesoderm
Tissue Culture Techniques
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Molecular Biology
Cell Shape
Lung
030304 developmental biology
Basement membrane
0303 health sciences
Mesenchymal stem cell
Tenascin C
Tenascin
Epithelium
Matrix Metalloproteinases
Cell biology
Body Fluids
Extracellular Matrix
medicine.anatomical_structure
biology.protein
Respiratory epithelium
030217 neurology & neurosurgery
Developmental Biology
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Development
- Accession number :
- edsair.doi.dedup.....c62c11247d35e547b3633007c117797d