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Evaluation of a New Culture Protocol for Enhancing Fungal Detection Rates in Respiratory Samples of Cystic Fibrosis Patients
- Source :
- Journal of Fungi, 6, 2, Journal of Fungi, Vol 6, Iss 82, p 82 (2020), Journal of Fungi, 6, Journal of Fungi, Volume 6, Issue 2
- Publication Year :
- 2020
-
Abstract
- Cystic fibrosis (CF) can be complicated by fungal infection of the respiratory tract. Fungal detection rates in CF sputa are highly dependent on the culture protocol and incubation conditions and thus may lead to an underestimation of the true prevalence of fungal colonization. We conducted a prospective study to evaluate the additional value of mucolytic pre-treatment, increased inoculum (100 &micro<br />L), additional fungal culture media (Sabouraud agar<br />SAB, Medium B+, Scedosporium selective agar<br />SceSel+ and Dichloran-Glycerol agar<br />DG18) and longer incubation time (3 weeks) compared with our current protocol. Using the new protocol, we prospectively analyzed 216 expectorated sputum samples from adult and pediatric CF patients (n = 77) and compared the culture yield to a three year retrospective cohort that used direct 10 &micro<br />L loop inoculation on SAB with 5 days incubation (867 sputum samples/103 patients). Detection rates for molds increased from 42% to 76% (p &lt<br />0.0001). Twenty-six percent of cultures were polymicrobial in the prospective cohort as opposed to 4.7% in the retrospective cohort (p &lt<br />0.0001). Colonization rate with A. fumigatus increased from 36% to 57%. SAB and DG18 showed the highest detection rates for all molds (SAB 58.6%<br />DG18 56.9%) and DG18 had the best performance for molds other than A. fumigatus. The larger sample volume and longer incubation also contributed to the increased recovery of molds. The introduction of a modified fungal culture protocol leads to a major increase in detection rate and the diversity of molds, which influences fungal epidemiology and may have implications for treatment decisions.
- Subjects :
- Microbiology (medical)
food.ingredient
DG18
culture protocol
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
Plant Science
Sabouraud agar
Cystic fibrosis
Article
Incubation period
Microbiology
cystic fibrosis
03 medical and health sciences
chemistry.chemical_compound
food
medicine
Agar
Prospective cohort study
Incubation
lcsh:QH301-705.5
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
0303 health sciences
Women's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17]
030306 microbiology
business.industry
aspergillus
Retrospective cohort study
medicine.disease
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
chemistry
lcsh:Biology (General)
Inflammatory diseases Radboud Institute for Health Sciences [Radboudumc 5]
Sputum
epidemiology
fungi
medicine.symptom
business
Subjects
Details
- ISSN :
- 2309608X
- Database :
- OpenAIRE
- Journal :
- Journal of Fungi, 6, 2, Journal of Fungi, Vol 6, Iss 82, p 82 (2020), Journal of Fungi, 6, Journal of Fungi, Volume 6, Issue 2
- Accession number :
- edsair.doi.dedup.....c652a201add5cdfccdb6f4646d0192f2