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New substituted aryldiazomethyl labels for high density DNA Chipbased nucleic acid testing
- Source :
- Nucleic Acids Symposium Series. 52:253-254
- Publication Year :
- 2008
- Publisher :
- Oxford University Press (OUP), 2008.
-
Abstract
- DNA and RNA labeling and detection are key steps in nucleic acid testing, particularly for molecular diagnostics applications. Here, we report a new class of aryldiazomethyl labels that include in their structure a biotin moiety as a detectable unit and a nitro substituted phenyl diazomethyl as a reactive group. The greatest reactivity towards phosphates of nucleic acids, the water solubility and the stability of these new molecules were demonstrated. These very important properties, which are the main requirements for nucleic acid labeling in aqueous conditions using automated protocols within integrated diagnostic devices, make them the perfect labeling tools for hybridization-based analysis, especially for high-density DNA chips.
- Subjects :
- Xeno nucleic acid
Nucleic acid quantitation
Oligonucleotide
Nucleic acid methods
Biotin
Diazonium Compounds
General Medicine
Biology
Nucleic acid thermodynamics
chemistry.chemical_compound
chemistry
Biochemistry
Molecular Probes
RNA, Ribosomal, 16S
Nucleic acid
Nucleic Acid Amplification Tests
DNA
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 17468272 and 02613166
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Symposium Series
- Accession number :
- edsair.doi.dedup.....c679c8e0a2c45599f1aea033306340e5
- Full Text :
- https://doi.org/10.1093/nass/nrn128