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Wnt/β-catenin signaling has an essential role in the initiation of limb regeneration
- Source :
- Developmental Biology. (1):170-178
- Publisher :
- Elsevier Inc.
-
Abstract
- Anuran (frog) tadpoles and urodeles (newts and salamanders) are the only vertebrates capable of fully regenerating amputated limbs. During the early stages of regeneration these amphibians form a "blastema", a group of mesenchymal progenitor cells that specifically directs the regrowth of the limb. We report that wnt-3a is expressed in the apical epithelium of regenerating Xenopus laevis limb buds, at the appropriate time and place to play a role during blastema formation. To test whether Wnt/beta-catenin signaling is required for limb regeneration, we created transgenic X. laevis tadpoles that express Dickkopf-1 (Dkk1), a specific inhibitor of Wnt/beta-catenin signaling, under the control of a heat-shock promoter. Heat-shock immediately before limb amputation or during early blastema formation blocked limb regeneration but did not affect the development of contralateral, un-amputated limb buds. When the transgenic tadpoles were heat-shocked following the formation of a blastema, however, they retained the ability to regenerate partial hindlimb structures. Furthermore, heat-shock induced Dkk1 blocked fgf-8 but not fgf-10 expression in the blastema. We conclude that Wnt/beta-catenin signaling has an essential role during the early stages of limb regeneration, but is not absolutely required after blastema formation.
- Subjects :
- animal structures
Fibroblast Growth Factor 8
Limb Buds
Xenopus
Hindlimb
Xenopus Proteins
Limb
Article
Transgenic
Animals, Genetically Modified
Wnt3 Protein
Xenopus laevis
Wnt3A Protein
Animals
Regeneration
HSP70 Heat-Shock Proteins
Promoter Regions, Genetic
Molecular Biology
beta Catenin
biology
Regeneration (biology)
Wnt signaling pathway
Extremities
Cell Biology
biology.organism_classification
Molecular biology
Cell biology
Wnt Proteins
body regions
fgf-10
DKK1
Catenin
Intercellular Signaling Peptides and Proteins
fgf-8
Fibroblast Growth Factor 10
Blastema
Signal Transduction
Developmental Biology
Wnt/β-catenin signaling
Subjects
Details
- Language :
- English
- ISSN :
- 00121606
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Developmental Biology
- Accession number :
- edsair.doi.dedup.....c684a98202b6c5139a60a332132fc6e7
- Full Text :
- https://doi.org/10.1016/j.ydbio.2007.03.014