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Decreased hepatic nitric oxide production contributes to the development of rat sinusoidal obstruction syndrome

Authors :
Margaret K. McCuskey
Zoltán A. Tökés
Xiangdong Wang
Jeffrey Tsai
Laurie D. DeLeve
Robert S. McCuskey
Nancy W. Bethea
Gary Kanel
Yoshiya Ito
Source :
Hepatology. 38:900-908
Publication Year :
2003
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2003.

Abstract

This study examined the role of decreased nitric oxide (NO) in the microcirculatory obstruction of hepatic sinusoidal obstruction syndrome (SOS). SOS was induced in rats with monocrotaline. Monocrotaline caused hepatic vein NO to decrease by 30% at 24 hours and by 70% at 72 hours; this decrease persisted throughout late SOS. N(G)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NO synthase, exacerbated monocrotaline toxicity, whereas V-PYRRO/NO, a liver-selective NO donor prodrug, restored NO levels, preserved sinusoidal endothelial cell (SEC) integrity and sinusoidal perfusion as assessed by in vivo microscopy and electron microscopy, and prevented clinical and histologic evidence of SOS. NO production in vitro by SEC and Kupffer cells, the 2 major liver cell sources of NO, decreases largely in parallel with loss of cell viability after exposure to monocrotaline. Increased matrix metalloproteinase (MMP) activity increases early on in SOS and this increase in activity has been implicated in initiating SOS. Infusion of V-PYRRO-NO prevented the monocrotaline-induced increase in MMP-9. In conclusion, decreased hepatic NO production contributes to the development of SOS. Infusion of an NO donor preserves SEC integrity and prevents development of SOS. These findings show that a decrease in NO contributes to SOS by allowing up-regulation of MMP activity, loss of sinusoidal integrity, and subsequent disruption of sinusoidal perfusion.

Details

ISSN :
02709139
Volume :
38
Database :
OpenAIRE
Journal :
Hepatology
Accession number :
edsair.doi.dedup.....c6cc1d65d7776222ff763d408e4bf445
Full Text :
https://doi.org/10.1053/jhep.2003.50383