Back to Search
Start Over
Permeability of membranes in the liquid ordered and liquid disordered phases
- Source :
- Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019), NATURE COMMUNICATIONS, Nature Communications
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The functional significance of ordered nanodomains (or rafts) in cholesterol rich eukaryotic cell membranes has only begun to be explored. This study exploits the correspondence of cellular rafts and liquid ordered (Lo) phases of three-component lipid bilayers to examine permeability. Molecular dynamics simulations of Lo phase dipalmitoylphosphatidylcholine (DPPC), dioleoylphosphatidylcholine (DOPC), and cholesterol show that oxygen and water transit a leaflet through the DOPC and cholesterol rich boundaries of hexagonally packed DPPC microdomains, freely diffuse along the bilayer midplane, and escape the membrane along the boundary regions. Electron paramagnetic resonance experiments provide critical validation: the measured ratio of oxygen concentrations near the midplanes of liquid disordered (Ld) and Lo bilayers of DPPC/DOPC/cholesterol is 1.75 ± 0.35, in very good agreement with 1.3 ± 0.3 obtained from simulation. The results show how cellular rafts can be structurally rigid signaling platforms while remaining nearly as permeable to small molecules as the Ld phase.<br />Increasing evidence supports the existence of ordered nanodomains (or rafts) in cholesterol rich cell membranes. Here authors present molecular dynamics simulations and EPR experiments to monitor permeation of oxygen and water through membranes in the liquid ordered and liquid disordered phases.
- Subjects :
- Cell Membrane Permeability
Lipid Bilayers
General Physics and Astronomy
OXYGEN PERMEABILITY
01 natural sciences
law.invention
Diffusion
Molecular dynamics
chemistry.chemical_compound
PERMEATION
law
lcsh:Science
Lipid bilayer
Electron paramagnetic resonance
TEMPERATURE
RAFTS
Multidisciplinary
010304 chemical physics
LIPID-MEMBRANES
CHOLESTEROL
Bilayer
Permeation
DIFFUSION
Chemistry
Cholesterol
Membrane
Chemical physics
Phosphatidylcholines
Thermodynamics
lipids (amino acids, peptides, and proteins)
BILAYERS
DOMAINS
Materials science
1,2-Dipalmitoylphosphatidylcholine
Science
Biophysics
Genetics and Molecular Biology
010402 general chemistry
Article
General Biochemistry, Genetics and Molecular Biology
Oxygen permeability
0103 physical sciences
Computer Simulation
Probability
DYNAMICS SIMULATIONS
technology, industry, and agriculture
Biology and Life Sciences
General Chemistry
Computational biology and bioinformatics
0104 chemical sciences
Oxygen
chemistry
Dipalmitoylphosphatidylcholine
General Biochemistry
lcsh:Q
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....c6d19c51382f64bfe8a368652a7a85f1