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Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testing
- Source :
- European Journal of Clinical Microbiology & Infectious Diseases, and the EME Evaluation Program Collaborative, Skov, M N & Agergaard, C N 2021, ' Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testing ', European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, vol. 40, no. 8, pp. 1609–1622 . https://doi.org/10.1007/s10096-021-04195-5
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- This study compared standard of care testing (SOC) to BioFire® FilmArray® Pneumonia plus Panel (PNplus). PNplus detects 15 bacteria with semiquantitative log bin values, 7 antibiotic resistance markers, three atypical bacteria (AB), and eight viral classes directly from bronchoalveolar lavage-like specimens (BLS) and sputum-like specimens (SLS). Fifty-two laboratories from 13 European countries and Israel tested 1234 BLS and 1242 SLS with PNplus and SOC. Detection rates and number of pathogens/samples were compared for PNplus pathogens. PNplus bin values and SOC quantities were compared. Three thousand two hundred sixty-two bacteria in PNplus were detected by PNplus and/or SOC. SOC detected 57.1% compared to 95.8% for PNplus (p ≤ 0.0001). PNplus semiquantitative bin values were less than SOC, equal to SOC, or greater than SOC in 5.1%, 25.4%, and 69.6% of results, respectively. PNplus bin values were on average ≥ 1 log than SOC values (58.5% 1–2 logs; 11.0% 3–4 logs). PNplus identified 98.2% of MRSA and SOC 55.6%. SOC detected 73/103 AB (70.9%) and 134/631 viruses (21.2%). PNplus detected 93/103 AB (90.3%) and 618/631 viruses (97.9%) (p ≤ 0.0001). PNplus and SOC mean number of pathogens/samples were 1.99 and 1.44, respectively. All gram-negative resistance markers were detected. PNplus and SOC results were fully or partially concordant for 49.1% and 26.4% of specimens, respectively. PNplus was highly sensitive and detected more potential pneumonia pathogens than SOC. Semiquantification may assist in understanding pathogen significance. As PNplus generates results in approximately 1 h, PNplus has potential to direct antimicrobial therapy in near real time and improve antimicrobial stewardship and patient outcomes.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Veterinary medicine
Standard of care
Atypical bacteria
030106 microbiology
03 medical and health sciences
0302 clinical medicine
Anti-Infective Agents
Diagnosis
otorhinolaryngologic diseases
Humans
Medicine
030212 general & internal medicine
Israel
BioFire Pneumonia plus Panel
Bacteria
business.industry
Standard of Care
Pneumonia
HAP
General Medicine
medicine.disease
CAP
Highly sensitive
Europe
Infectious Diseases
Molecular Diagnostic Techniques
Viruses
VAP
Original Article
Detection rate
business
Subjects
Details
- ISSN :
- 14354373 and 09349723
- Database :
- OpenAIRE
- Journal :
- European Journal of Clinical Microbiology & Infectious Diseases
- Accession number :
- edsair.doi.dedup.....c7014d9ead93eeda1e14e1ae9c1d736d