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Genomic in situ hybridization in interspecific hybrids of scallops (Bivalvia, Pectinidae) and localization of the satellite DNA Cf303, and the vertebrate telomeric sequences (TTAGGG)n on chromosomes of scallop Chlamys farreri (Jones & Preston, 1904)
- Source :
- Comparative Cytogenetics, Vol 12, Iss 1, Pp 83-95 (2018)
- Publication Year :
- 2018
- Publisher :
- Pensoft Publishers, 2018.
-
Abstract
- Mitotic chromosome preparations of the interspecific hybrids Chlamysfarreri (Jones & Preston, 1904) × Patinopectenyessoensis (Jay, 1857), C.farreri × Argopectenirradinas (Lamarck, 1819) and C.farreri × Mimachlamysnobilis (Reeve, 1852) were used to compare two different scallop genomes in a single slide. Although genomic in situ hybridization (GISH) using genomic DNA from each scallop species as probe painted mitotic chromosomes of the interspecific hybrids, the painting results were not uniform; instead it showed species-specific distribution patterns of fluorescent signals among the chromosomes. The most prominent GISH-bands were mainly located at centromeric or telomeric regions of scallop chromosomes. In order to illustrate the sequence constitution of the GISH-bands, the satellite Cf303 sequences of C.farreri and the vertebrate telomeric (TTAGGG)n sequences were used to map mitotic chromosomes of C.farreri by fluorescence in situ hybridization (FISH). The results indicated that the GISH-banding pattern presented by the chromosomes of C.farreri is mainly due to the distribution of the satellite Cf303 DNA, therefore suggesting that the GISH-banding patterns found in the other three scallops could also be the result of the chromosomal distribution of other species-specific satellite DNAs.
- Subjects :
- 0301 basic medicine
lcsh:QH426-470
biology
medicine.diagnostic_test
Satellite DNA
Argopecten irradians
Patinopecten yessoensis
Plant Science
biology.organism_classification
Genome
lcsh:Genetics
03 medical and health sciences
genomic DNA
030104 developmental biology
Evolutionary biology
Scallop
Genetics
medicine
Animal Science and Zoology
Mitosis
Biotechnology
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 1993078X and 19930771
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Comparative Cytogenetics
- Accession number :
- edsair.doi.dedup.....c739cc2c865d872a656f651d3d9fbff0