Back to Search
Start Over
Zinc oxide nanoparticles inhibit expression of manganese superoxide dismutase via amplification of oxidative stress, in murine photoreceptor cells
- Source :
- Cell proliferation. 49(3)
- Publication Year :
- 2016
-
Abstract
- Objectives As a parenchymal cell, the photoreceptor is more susceptible to alterations in outer micro-environmental conditions than other cells. In the present study, we aimed to investigate inhibitory effects of zinc oxide (ZnO) nanoparticles on expression of manganese superoxide dismutase (MnSOD) in murine photoreceptor-derived cells. Materials and methods We investigated effects of ZnO nanoparticles on murine photoreceptor cell viability and on expression and activity of MnSOD using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, immunofluorescence analysis, flow cytometry, quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA). Results ZnO nanoparticles were found to have higher cytotoxic effects in concentration- and time-dependent manners, to elevate intracellular levels of hydrogen peroxide and hydroxyl radicals, and thus to induce overproduction of reactive oxygen species (ROS) and collapse of mitochondrial membrane potential, leading to cell damage. Moreover, ZnO nanoparticles also significantly reduced expression of MnSOD at both the mRNA and protein levels, reduced its activity, and further aggravated oxidative stress-mediated cell damage. Conclusion Overall, ZnO nanoparticle-induced cytotoxicity was associated with elevated levels of oxidative stress due to overproduction of ROS and reduced expression and activity of MnSOD.
- Subjects :
- 0301 basic medicine
Cell Survival
Down-Regulation
02 engineering and technology
Oxidative phosphorylation
medicine.disease_cause
Flow cytometry
Cell Line
03 medical and health sciences
Mice
medicine
Animals
Photoreceptor Cells
Cytotoxicity
Cell damage
chemistry.chemical_classification
Reactive oxygen species
medicine.diagnostic_test
Superoxide Dismutase
Cell Biology
General Medicine
Original Articles
021001 nanoscience & nanotechnology
medicine.disease
Molecular biology
Oxidative Stress
030104 developmental biology
chemistry
Cell culture
Nanoparticles
Zinc Oxide
0210 nano-technology
Reactive Oxygen Species
Intracellular
Oxidative stress
Subjects
Details
- ISSN :
- 13652184
- Volume :
- 49
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cell proliferation
- Accession number :
- edsair.doi.dedup.....c79b9036d40db575083f238db7a4e020