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Lack of trehalose accelerates H2O2-induced Candida albicans apoptosis through regulating Ca2+ signaling pathway and caspase activity
- Source :
- PLoS ONE, Vol 6, Iss 1, p e15808 (2011), PLoS ONE
- Publication Year :
- 2011
- Publisher :
- Public Library of Science (PLoS), 2011.
-
Abstract
- Trehalose is a non-reducing disaccharide and can be accumulated in response to heat or oxidative stresses in Candida albicans. Here we showed that a C. albicans tps1Δ mutant, which is deficient in trehalose synthesis, exhibited increased apoptosis rate upon H(2)O(2) treatment together with an increase of intracellular Ca(2+) level and caspase activity. When the intracellular Ca(2+) level was stimulated by adding CaCl(2) or A23187, both the apoptosis rate and caspase activity were increased. In contrast, the presence of two calcium chelators, EGTA and BAPTA, could attenuate these effects. Moreover, we investigated the role of Ca(2+) pathway in C. albicans apoptosis and found that both calcineurin and the calcineurin-dependent transcription factor, Crz1p, mutants showed decreased apoptosis and caspase activity upon H(2)O(2) treatment compared to the wild-type cells. Expression of CaMCA1, the only gene found encoding a C. albicans metacaspase, in calcineurin-deleted or Crz1p-deleted cells restored the cell sensitivity to H(2)O(2). Our results suggest that Ca(2+) and its downstream calcineurin/Crz1p/CaMCA1 pathway are involved in H(2)O(2)-induced C. albicans apoptosis. Inhibition of this pathway might be the mechanism for the protective role of trehalose in C. albicans.
- Subjects :
- Infectious Disease Control
lcsh:Medicine
Apoptosis
Mycology
Microbiology
Signaling Pathways
chemistry.chemical_compound
Systemic Mycoses
BAPTA
Candida albicans
Molecular Cell Biology
Calcium-Mediated Signal Transduction
Calcium Signaling
lcsh:Science
Biology
Microbial Pathogens
Caspase
Cellular Stress Responses
Multidisciplinary
biology
Cell Death
Calcineurin
lcsh:R
Fungi
Microbial Growth and Development
Fungal Diseases
Trehalose
Hydrogen Peroxide
biology.organism_classification
Molecular biology
Corpus albicans
Yeast
Cell biology
EGTA
Oxidative Stress
Infectious Diseases
chemistry
Medical Microbiology
Caspases
Mutation
biology.protein
Medicine
lcsh:Q
Intracellular
Research Article
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....c7a92e7628cb3b43794071c0700ce68e