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Expression of Glutamate Transporters in Mouse Liver, Kidney, and Intestine
- Source :
- Journal of Histochemistry & Cytochemistry. 66:189-202
- Publication Year :
- 2018
- Publisher :
- SAGE Publications, 2018.
-
Abstract
- Glutamate transport activities have been identified not only in the brain, but also in the liver, kidney, and intestine. Although glutamate transporter distributions in the central nervous system are fairly well known, there are still uncertainties with respect to the distribution of these transporters in peripheral organs. Quantitative information is mostly lacking, and few of the studies have included genetically modified animals as specificity controls. The present study provides validated qualitative and semi-quantitative data on the excitatory amino acid transporter (EAAT)1–3 subtypes in the mouse liver, kidney, and intestine. In agreement with the current view, we found high EAAT3 protein levels in the brush borders of both the distal small intestine and the renal proximal tubules. Neither EAAT1 nor EAAT2 was detected at significant levels in murine kidney or intestine. In contrast, the liver only expressed EAAT2 (but 2 C-terminal splice variants). EAAT2 was detected in the plasma membranes of perivenous hepatocytes. These cells also expressed glutamine synthetase. Conditional deletion of hepatic EAAT2 did neither lead to overt neurological disturbances nor development of fatty liver.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Histology
Immunoblotting
Central nervous system
Biology
Kidney
Mice
03 medical and health sciences
0302 clinical medicine
Internal medicine
Glutamine synthetase
medicine
Animals
Staining and Labeling
Fatty liver
Glutamate receptor
Transporter
Articles
medicine.disease
Immunohistochemistry
Small intestine
Excitatory Amino Acid Transporter 1
Intestines
Excitatory Amino Acid Transporter 3
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Excitatory Amino Acid Transporter 2
Liver
Hepatocyte
Anatomy
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15515044 and 00221554
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Journal of Histochemistry & Cytochemistry
- Accession number :
- edsair.doi.dedup.....c7c2bc1bcbdb39b3afeda9944b256dda