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Quantitative Analysis of Fentanyl and Six Fentanyl Analogs in Postmortem Specimens by UHPLC–MS-MS†
- Source :
- Journal of Analytical Toxicology. 42:570-580
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Since 2013, opioid-related deaths have increased in Miami-Dade County, FL due to fentanyl and fentanyl analogs in the local heroin supply. From 2014 to 2015, a near 600% increase in fentanyl and fentanyl analog-related deaths was observed, followed by another 200% increase in 2016. In addition to fentanyl, six fentanyl analogs were identified in this time period: β-hydroxythiofentanyl, acetyl fentanyl, furanyl fentanyl, carfentanil, butyryl fentanyl and para-fluoroisobutyryl fentanyl. As a result, an analytical method to quantify these compounds in postmortem biological fluids and tissues using solid-phase extraction with analysis by ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS-MS) was developed and validated. All analytes except carfentanil were linear from 1 to 50 ng/mL with a limit of detection (LOD) of 0.5 ng/mL; carfentanil was linear from 0.2 to 10 ng/mL with a LOD of 0.1 ng/mL. For all analytes, bias was within ±14%, intra- and inter-day precision were ≤5% and extraction efficiency was >87%. Dilution integrity was assessed at 1:1, 1:4 and 1:9 (v/v) ratios; percent error from target was
- Subjects :
- Health, Toxicology and Mutagenesis
Toxicology
Tandem mass spectrometry
01 natural sciences
Uhplc ms ms
Analytical Chemistry
Carfentanil
Fentanyl
Forensic Toxicology
03 medical and health sciences
0302 clinical medicine
Limit of Detection
Tandem Mass Spectrometry
medicine
Humans
Environmental Chemistry
030216 legal & forensic medicine
Solid phase extraction
Chromatography, High Pressure Liquid
Whole blood
Detection limit
Chemical Health and Safety
Chromatography
Illicit Drugs
Chemistry
Solid Phase Extraction
010401 analytical chemistry
Reproducibility of Results
0104 chemical sciences
Substance Abuse Detection
Postmortem Changes
Quantitative analysis (chemistry)
medicine.drug
Subjects
Details
- ISSN :
- 19452403 and 01464760
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Journal of Analytical Toxicology
- Accession number :
- edsair.doi.dedup.....c7f046d6968f02935f24a035f8126e23
- Full Text :
- https://doi.org/10.1093/jat/bky054