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Adhesive Hierarchy of the Cell Adhesion Molecules L1, CD24, and α6 Integrin in Murine Neuroblastoma N2A Cells
- Source :
- Biochemical and Biophysical Research Communications. 214:94-101
- Publication Year :
- 1995
- Publisher :
- Elsevier BV, 1995.
-
Abstract
- The aggregation rate of resuspended neuroblastoma N2A cells depends on the density of the cells in culture prior to their resuspension: isolated, fast growing cells have a weak tendency to aggregate whereas confluent, slowly growing cells reaggregate very strongly. L1 antibody 557 strongly inhibited the slow aggregation of isolated, fast growing cells but not the reaggregation of confluent cells. CD24 (nectadrin) antibodies did not affect the aggregation of isolated or confluent cells but stimulated the aggregation of subconfluent cells. In all stages aggregation was not inhibited when antibody 557 was used together with CD24 antibodies at 37 degrees C in the presence of divalent cations. EA-1 antibody to alpha 6 integrin chain stimulated the aggregation of subconfluent cells but inhibited the reaggregation of confluent cells. Therefore, L1 appears to be an early recognition molecule mediating weak primary adhesion. CD24 appears to participate in activating secondary adhesion mechanisms during primary adhesion, possibly in cooperation with L1, and alpha 6 integrin seems to serve as a secondary, strong adhesion molecule that in early adhesion phases also mediates the activation of itself or of other adhesion mechanisms. These results indicate that neural cells might employ a strategy of adhesion cascade in establishing stable contacts.
- Subjects :
- Integrins
Cell Adhesion Molecules, Neuronal
Biophysics
Integrin alpha6
Biochemistry
Antibodies
Divalent
Mice
Antigens, CD
Tumor Cells, Cultured
Animals
Cell adhesion
Molecular Biology
Cell Aggregation
chemistry.chemical_classification
Confluency
Membrane Glycoproteins
biology
Chemistry
Cell adhesion molecule
CD24
CD24 Antigen
Cell Biology
Adhesion
Cell biology
biology.protein
Neural cell adhesion molecule
Antibody
Cell Adhesion Molecules
Leukocyte L1 Antigen Complex
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 214
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....c82ce40c00e9d84f6f9d0427facce8ea