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Metabolism and antioxidation regulation of total flavanones from Sedum sarmentosum Bunge against high-fat diet-induced fatty liver disease in Nile tilapia (Oreochromis niloticus)
- Source :
- Fish Physiology and Biochemistry. 47:1149-1164
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Diet-induced fatty liver is a considerable threaten to fish aquaculture due to the popularity of the high-fat diet (HFD) feeding. Our study aims to investigate the effects of flavanones from Sedum sarmentosum Bunge (FSSB) on the liver function to identify a potential treatment for HFD-induced fatty liver disease. Physiological and pathological indicators were tested in the liver of Nile tilapia (Oreochromis niloticus) and results showed parameters including lipid metabolites, redox parameters, and inflammatory factors could be adequately restored to normal level by addition of 150 mg/kg FSSB to HFD. Proteomics analysis was performed in liver tissues from tilapia with normal diet (ND), HFD, and HFD+FSSB. Totally, 51 upregulated proteins and 77 downregulated proteins were identified in HFD groups and 67 proteins of them were restored after treated with FSSB. Bioinformatics analysis showed that differentially expressed proteins (DEPs) in HFD+FSSB150 group compared with HFD group are mainly enriched in acety-CoA metabolic process, adenosine-triphosphate (ATP) biosynthetic process, lipid metabolic process, and phospholipid metabolic process. The dysregulated proteins were involved in peroxidosome proliferators-activated receptor (PPAR) signaling pathway, fat digestion and absorption, and immune system. The quantitative real-time PCR (qRT-PCR) assay further revealed that the expression of GST, PPARĪ±, PPARĪ³, and multiple-inflammatory cytokines could be also reversed in HFD group under the treatment of 150 mg/kg FSSB. Our findings demonstrated FSSB is efficient for the treatment of fatty liver disease through regulation of lipid metabolism and antioxidation in Nile tilapia, providing a new treatment of non-alcoholic fatty liver disease (NAFLD) in fish aquaculture.
- Subjects :
- Fish Proteins
medicine.medical_specialty
Normal diet
Physiology
Aquatic Science
Biology
Diet, High-Fat
Biochemistry
Antioxidants
Sedum
Fish Diseases
03 medical and health sciences
Nile tilapia
Internal medicine
medicine
Animals
Phospholipid metabolic process
PPAR alpha
Triglycerides
Glutathione Transferase
030304 developmental biology
0303 health sciences
Fatty liver
Lipid metabolism
Cichlids
04 agricultural and veterinary sciences
General Medicine
Lipid Metabolism
medicine.disease
biology.organism_classification
Fatty Liver
PPAR gamma
Oreochromis
Cholesterol
Endocrinology
Liver
Biosynthetic process
Flavanones
Hepatocytes
040102 fisheries
0401 agriculture, forestry, and fisheries
lipids (amino acids, peptides, and proteins)
Liver function
Subjects
Details
- ISSN :
- 15735168 and 09201742
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Fish Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....c83b82e8edbaaa91ed50b36ea9252a18