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A polysaccharide from Eclipta prostrata alleviates experimental autoimmune encephalomyelitis through inhibiting Th17 cells
- Source :
- Carbohydrate Polymers. 201:608-614
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Eclipta prostrata has long been used as a medicinal herb in China. EAP20-1, a homogeneous polysaccharide with anti-complementary activity had been obtained from E. prostrate by using anion-exchange and size-exclusion chromatography. In this study, we found that EAP20-1 could inhibit in vitro lymphocyte proliferation stimulated by concanavalin–A or anti-CD3/anti-CD28 antibodies. Furthermore, in experimental autoimmune encephalomyelitis (EAE) mice, EAP20-1 treatment relieved the clinical symptoms, accompanied by reduced neuroinflammation and demyelination in spinal cords. Mechanistically, EAP20-1 reduced the mRNA expression of interleukin (IL)-17, IL-22, and RAR-related orphan receptor gamma t (RORγt) in the spleen; inhibited auto-reactive T cell proliferation and decreased the percentage of Th17 cells in response to myelin oligodendrocyte glycoprotein (MOG35-55) ex vivo. Moreover, EAP20-1 directly inhibited naive CD4 + T cells differentiate into Th17 cells in vitro. These results indicating EAP20-1 could benefit EAE through inhibiting Th17 cell differentiation and suggesting a therapeutic potential of EAP20-1 in MS.
- Subjects :
- 0301 basic medicine
Encephalomyelitis, Autoimmune, Experimental
Polymers and Plastics
T cell
Lymphocyte proliferation
Pharmacology
Myelin oligodendrocyte glycoprotein
Mice
03 medical and health sciences
0302 clinical medicine
Polysaccharides
RAR-related orphan receptor gamma
Materials Chemistry
medicine
Animals
Neuroinflammation
Eclipta prostrata
Cell Proliferation
Mice, Inbred BALB C
biology
Chemistry
Interleukins
Interleukin-17
Organic Chemistry
Experimental autoimmune encephalomyelitis
Interleukin
Cell Differentiation
Eclipta
Nuclear Receptor Subfamily 1, Group F, Member 3
medicine.disease
biology.organism_classification
030104 developmental biology
medicine.anatomical_structure
biology.protein
Th17 Cells
Female
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 01448617
- Volume :
- 201
- Database :
- OpenAIRE
- Journal :
- Carbohydrate Polymers
- Accession number :
- edsair.doi.dedup.....c845b16657e7cef9bc3d8dc47dc1059b