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Selective Ablation of the Androgen Receptor in Mouse Sertoli Cells Affects Sertoli Cell Maturation, Barrier Formation and Cytoskeletal Development
- Source :
- PLoS ONE, Willems, A, Batlouni, S R, Esnal, A, Swinnen, J V, Saunders, P T K, Sharpe, R M, França, L R, De Gendt, K & Verhoeven, G 2010, ' Selective ablation of the androgen receptor in mouse sertoli cells affects sertoli cell maturation, barrier formation and cytoskeletal development ', PLoS ONE, vol. 5, no. 11, e14168 . https://doi.org/10.1371/journal.pone.0014168, PLoS ONE, Vol 5, Iss 11, p e14168 (2010), Web of Science, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2010
- Publisher :
- Public Library of Science, 2010.
-
Abstract
- Made available in DSpace on 2013-08-28T14:10:27Z (GMT). No. of bitstreams: 1 WOS000284755100045.pdf: 7773713 bytes, checksum: 446d5dca1e03fd0b4dbdedf58817301b (MD5) Made available in DSpace on 2013-09-30T19:22:37Z (GMT). No. of bitstreams: 2 WOS000284755100045.pdf: 7773713 bytes, checksum: 446d5dca1e03fd0b4dbdedf58817301b (MD5) WOS000284755100045.pdf.txt: 88228 bytes, checksum: 90fdef08d8046412e8a8ad044de99c98 (MD5) Previous issue date: 2010-11-30 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:33:53Z No. of bitstreams: 2 WOS000284755100045.pdf: 7773713 bytes, checksum: 446d5dca1e03fd0b4dbdedf58817301b (MD5) WOS000284755100045.pdf.txt: 88228 bytes, checksum: 90fdef08d8046412e8a8ad044de99c98 (MD5) Made available in DSpace on 2014-05-20T15:33:53Z (GMT). No. of bitstreams: 2 WOS000284755100045.pdf: 7773713 bytes, checksum: 446d5dca1e03fd0b4dbdedf58817301b (MD5) WOS000284755100045.pdf.txt: 88228 bytes, checksum: 90fdef08d8046412e8a8ad044de99c98 (MD5) Previous issue date: 2010-11-30 Katholieke Universiteit Leuven Fund for Scientific Research Flanders (FWO) Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) The observation that mice with a selective ablation of the androgen receptor (AR) in Sertoli cells (SC) (SCARKO mice) display a complete block in meiosis supports the contention that SC play a pivotal role in the control of germ cell development by androgens. To delineate the physiological and molecular mechanism responsible for this control, we compared tubular development in pubertal SCARKO mice and littermate controls. Particular attention was paid to differences in SC maturation, SC barrier formation and cytoskeletal organization and to the molecular mediators potentially involved. Functional analysis of SC barrier development by hypertonic perfusion and lanthanum permeation techniques and immunohistochemical analysis of junction formation showed that SCARKO mice still attempt to produce a barrier separating basal and adluminal compartment but that barrier formation is delayed and defective. Defective barrier formation was accompanied by disturbances in SC nuclear maturation (immature shape, absence of prominent, tripartite nucleoli) and SC polarization (aberrant positioning of SC nuclei and cytoskeletal elements such as vimentin). Quantitative RT-PCR was used to study the transcript levels of genes potentially related to the described phenomena between day 8 and 35. Differences in the expression of SC genes known to play a role in junction formation could be shown from day 8 for Cldn11, from day 15 for Cldn3 and Espn, from day 20 for Cdh2 and Jam3 and from day 35 for ZO-1. Marked differences were also noted in the transcript levels of several genes that are also related to cell adhesion and cytoskeletal dynamics but that have not yet been studied in SC (Actn3, Ank3, Anxa9, Scin, Emb, Mpzl2). It is concluded that absence of a functional AR in SC impedes the remodeling of testicular tubules expected at the onset of spermatogenesis and interferes with the creation of the specific environment needed for germ cell development. Katholieke Univ Leuven, Lab Expt Med & Endocrinol, Louvain, Belgium São Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, Brazil MRC, Ctr Reprod Biol, Human Reprod Sci Unit, Edinburgh, Midlothian, Scotland Univ Fed Minas Gerais, Lab Cellular Biol, Dept Morphol, Inst Biol Sci, Belo Horizonte, MG, Brazil São Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, Brazil Katholieke Universiteit Leuven: OT/07/068A Fund for Scientific Research Flanders (FWO): G.0458.05N Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen): SB-73064
- Subjects :
- Male
Time Factors
Cellular differentiation
Genetics and Genomics/Nuclear Structure and Function
lcsh:Medicine
Gene Expression
IMMUNOGLOBULIN SUPERFAMILY
Mice
BLOOD-TESTIS BARRIER
Testis
Claudin-3
Receptor
Cytoskeleton
lcsh:Science
Mice, Knockout
Multidisciplinary
Tight junction
Reverse Transcriptase Polymerase Chain Reaction
BINDING PROTEINS
Genetics and Genomics/Gene Expression
Cell Differentiation
Sertoli cell
Cadherins
Immunohistochemistry
Cell biology
Physiology/Reproductive Physiology
Multidisciplinary Sciences
medicine.anatomical_structure
Receptors, Androgen
Science & Technology - Other Topics
Female
TESTICULAR GENE-EXPRESSION
Germ cell
Research Article
Genetics and Genomics/Animal Genetics
MUTANT MOUSE
Nerve Tissue Proteins
Biology
POSTNATAL-DEVELOPMENT
medicine
Animals
Vimentin
FUNCTIONAL MATURATION
Cell adhesion
Spermatogenesis
Science & Technology
Sertoli Cells
Physiology/Endocrinology
lcsh:R
Membrane Proteins
MALE-MICE LACKING
FOLLICLE-STIMULATING-HORMONE
Phosphoproteins
GERM-CELL
Androgen receptor
Mice, Inbred C57BL
Microscopy, Electron
Claudins
Zonula Occludens-1 Protein
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 5
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....c8471bf05039f41a9a1298299ec7a775