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Toll-like receptor 2 mediates invasion via activating NF-κB in MDA-MB-231 breast cancer cells
- Source :
- Biochemical and Biophysical Research Communications. 379:1027-1032
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- MDA-MB-231 breast cancer cells have a high invasive potential, yet the mechanisms involved are not known. This study showed that Toll-like receptor 2 (TLR2) was highly expressed in MDA-MB-231 cells and played a critical role in cell invasion. Compared with the poorly invasive MCF-7 cells, MDA-MB-231 cells expressed 10.5-fold more TLR2. Using TLR2 agonist pg-LPS and TLR2 neutralizing antibody, we found that TLR2 activation significantly promoted MDA-MB-231 invasion, whereas TLR2 blockade diminished this capacity. TLR2 activation enhanced the activity of NF-kappaB and induced phosphorylation of TAK1 and IkappaBalpha in the TLR2/NF-kappaB signaling pathway in MDA-MB-231, but not in MCF-7 cells. TLR2 activation increased IL-6, TGF-beta, VEGF and MMP9 secretion, which are associated with TLR2-NF-kappaB signaling. We demonstrated that TLR2 is a critical receptor responsible for NF-kappaB signaling activity and highly invasive capacity of MDA-MB-231 cells.
- Subjects :
- Vascular Endothelial Growth Factor A
Biophysics
Breast Neoplasms
Biochemistry
chemistry.chemical_compound
Transforming Growth Factor beta
Cell Line, Tumor
Humans
Neoplasm Invasiveness
skin and connective tissue diseases
Receptor
Molecular Biology
Toll-like receptor
biology
Interleukin-6
Chemistry
NF-kappa B
NF-κB
Cell Biology
Transforming growth factor beta
Toll-Like Receptor 2
Cell biology
TLR2
Matrix Metalloproteinase 9
Cell culture
biology.protein
Phosphorylation
Signal transduction
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 379
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....c89c7bf97f118ef6a2891a34d2feaab1