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Chinese medicine Bazi Bushen capsule improves lipid metabolism in ovariectomized female ApoE-/- mice
- Source :
- Annals of palliative medicine. 9(3)
- Publication Year :
- 2020
-
Abstract
- Background Dyslipidemia is one of the mechanisms of atherosclerosis (AS). Depletion of estrogen plays a key role in the pathogenesis of postmenopausal AS in women, and the blood lipid levels of women are closely related to endogenous estrogen levels. Phytoestrogens (PEs) exert estrogenic effects, including protection against AS, without the adverse effects of estrogen administration. Bazi Bushen capsule (BZBS) is a traditional Chinese medicine herbal compound prescription that has been shown to contain 11 unique PEs. In the present study, we assessed the effects of BZBS against lipid metabolism disorders. Methods All ApoE-/- mice underwent ovary ligation and bilateral ovariectomy (Ovx) to induce surgical menopause (Ovx/ApoE-/- mice), whereas the C57BL/6J mice underwent sham surgery (needle threading). Ovx/ApoE-/- mice were given a high-fat diet without estrogen and C57BL/6J mice were given a normal diet for 12 weeks. Ovx/ApoE-/- mice treated with G1, a highly selective G-protein-coupled estrogen receptor1 (GPER1) agonist with proven activity against AS, were used as positive controls. Estrogen levels were measured and uterine atrophy index was calculated to determine the success of the model. Serum levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were measured in each group. The orthogonal projections to latent structures discriminant analysis (OPLS-DA) model was used to separate the groups, MetaboAnalyst was then used to analyze the metabolic pathway, and the most representative metabolites were finally identified. Results Removal of bilateral ovaries resulted in successful surgical menopause models, where BZBS increased estrogen levels but did not increase the risk of uterine proliferation. BZBS attenuated dyslipidemia, including decreased TG, TC, and LDL-C levels, but increased HDL-C levels. The OPLS-DA model successfully distinguished the groups with good predictive ability and revealed their tendency to separate from each other. MetaboAnalyst suggested that both the G1 group and high-dose BZBS (HD-BZ) could improve the effect of lipid metabolism: the glycerophospholipid metabolism pathway was mainly improved by the G1 group, while the inositol phosphate metabolism pathway was mainly improved by the HD-BZ group. For the four compounds with the highest content, the concentrations of docosahexaenoic acid (DHA), 3-hydroxybutyric acid, and 5(Z), 8(Z), 11(Z)-eicosatrienoic acid were dramatically lower in the model group compared to the control group. Lysophosphatidylethanolamine (18:0) was higher in the model group than in the control group. BZBS corrected these effects. Conclusions BZBS treatment reduced serum lipid levels and improved fatty acid metabolism in high-fat diet-fed, surgically induced menopausal ApoE-/- mice.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Normal diet
medicine.drug_class
Blood lipids
030204 cardiovascular system & hematology
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Apolipoproteins E
Internal medicine
medicine
Animals
Humans
Medicine, Chinese Traditional
Advanced and Specialized Nursing
medicine.diagnostic_test
Fatty acid metabolism
business.industry
Lipid metabolism
medicine.disease
Atherosclerosis
Lipid Metabolism
Mice, Inbred C57BL
030104 developmental biology
Anesthesiology and Pain Medicine
Endocrinology
chemistry
Estrogen
Phytoestrogens
Female
Lipid profile
business
Dyslipidemia
Subjects
Details
- ISSN :
- 22245839
- Volume :
- 9
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Annals of palliative medicine
- Accession number :
- edsair.doi.dedup.....c8abc2eb639d93be28849cd9b67169df