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MiR-455-5p acts as a novel tumor suppressor in gastric cancer by down-regulating RAB18
- Source :
- Gene. 592:308-315
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Aim To detect the potential regulation pathway of microRNA-455-5p (miR-455-5p) and RAB18, member RAS oncogene family (RAB18) in gastric cancer (GC) cells and tissues and discuss the clinical significance of miR-455-5p in GC genesis and progression. Methods Real-time PCR was used to measure mRNA level of miR-455-5p in GC, TargetScan and dual luciferase assay were used to predict and demonstrate the candidate target gene of miR-455-5p, Western blot were utilized to detect the protein level of RAB18. Cell function assays were also performed to determine the function of miR-455-5p in GC. Results miR-455-5p was reduced significantly in gastric cancer cells and tissues compared with the corresponding normal control, the lower expression of miR-455-5p was related to advanced clinical stage in gastric cancer, re-expression of miR-455-5p could inhibit human GC cell proliferation and invasion, overexpression of miR-455-5p could also promote GC cell apoptosis. Furthermore, we found that RAB18 was a candidate target of miR-455-5p. Re-expression of miR-455-5p could inhibit the protein level of RAB18. Conclusion MiR-455-5p might serve as a novel biomarker in gastric cancer by targeting RAB18.
- Subjects :
- Male
0301 basic medicine
Down-Regulation
Apoptosis
Biology
03 medical and health sciences
0302 clinical medicine
Western blot
Cell Movement
Stomach Neoplasms
Cell Line, Tumor
Biomarkers, Tumor
Genetics
medicine
Humans
Neoplasm Invasiveness
Luciferase
Oncogene
medicine.diagnostic_test
Cell growth
Cancer
General Medicine
Middle Aged
medicine.disease
Molecular biology
Biomarker (cell)
MicroRNAs
030104 developmental biology
rab GTP-Binding Proteins
030220 oncology & carcinogenesis
Cancer cell
Female
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 592
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....c8b5bf4962f0598b2669e9223de99b92