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Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation
- Source :
- The Journal of Cell Biology
- Publication Year :
- 2012
- Publisher :
- The Rockefeller University Press, 2012.
-
Abstract
- Rlp24 recruits Drg1 to pre-60S particles and stimulates its ATP hydrolysis to promote downstream maturation through specific extraction of Rlp24.<br />Formation of eukaryotic ribosomes is driven by energy-consuming enzymes. The AAA-ATPase Drg1 is essential for the release of several shuttling proteins from cytoplasmic pre-60S particles and the loading of late joining proteins. However, its exact role in ribosome biogenesis has been unknown. Here we show that the shuttling protein Rlp24 recruited Drg1 to pre-60S particles and stimulated its ATPase activity. ATP hydrolysis in the second AAA domain of Drg1 was required to release shuttling proteins. In vitro, Drg1 specifically and exclusively extracted Rlp24 from purified pre-60S particles. Rlp24 release required ATP and was promoted by the interaction of Drg1 with the nucleoporin Nup116. Subsequent ATP hydrolysis in the first AAA domain dissociated Drg1 from Rlp24, liberating both proteins for consecutive cycles of activity. Our results show that release of Rlp24 by Drg1 defines a key event in large subunit formation that is a prerequisite for progression of cytoplasmic pre-60S maturation.
- Subjects :
- Adenosine Triphosphatases
Ribosomal Proteins
0303 health sciences
Cytoplasm
Saccharomyces cerevisiae Proteins
Protein subunit
Hydrolysis
030302 biochemistry & molecular biology
Ribosome biogenesis
Cell Biology
Saccharomyces cerevisiae
Biology
Ribosome Subunits, Large, Eukaryotic
Ribosome
AAA proteins
Article
Cell biology
03 medical and health sciences
ATP hydrolysis
Ribosomal protein
Nucleoporin
CLPB
Research Articles
030304 developmental biology
Subjects
Details
- Language :
- English
- ISSN :
- 15408140 and 00219525
- Volume :
- 199
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....c8bcf4dcb071aa6b9aa234059543ab61